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Actin-dependent vacuolar occupancy of the cell determines auxin-induced growth repression

The cytoskeleton is an early attribute of cellular life, and its main components are composed of conserved proteins. The actin cytoskeleton has a direct impact on the control of cell size in animal cells, but its mechanistic contribution to cellular growth in plants remains largely elusive. Here, we...

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Main Authors: Scheuring, David (Author) , Löfke, Christian (Author) , Krüger, Falco (Author) , Kittelmann, Maike (Author) , Eisa, Ahmed (Author) , Hughes, Louise (Author) , Smith, Richard S. (Author) , Hawes, Chris (Author) , Schumacher, Karin (Author) , Kleine-Vehn, Jürgen (Author)
Format: Article (Journal)
Language:English
Published: 2016
In: Proceedings of the National Academy of Sciences of the United States of America
Year: 2015, Volume: 113, Issue: 2, Pages: 452-457
ISSN:1091-6490
DOI:10.1073/pnas.1517445113
Online Access:Resolving-System, lizenzpflichtig, Volltext: https://doi.org/10.1073/pnas.1517445113
Verlag, lizenzpflichtig, Volltext: https://www.pnas.org/content/113/2/452
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Author Notes:David Scheuring, Christian Löfke, Falco Krüger, Maike Kittelmann, Ahmed Eisa, Louise Hughes, Richard S. Smith, Chris Hawes, Karin Schumacher, and Jürgen Kleine-Vehn
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Summary:The cytoskeleton is an early attribute of cellular life, and its main components are composed of conserved proteins. The actin cytoskeleton has a direct impact on the control of cell size in animal cells, but its mechanistic contribution to cellular growth in plants remains largely elusive. Here, we reveal a role of actin in regulating cell size in plants. The actin cytoskeleton shows proximity to vacuoles, and the phytohormone auxin not only controls the organization of actin filaments but also impacts vacuolar morphogenesis in an actin-dependent manner. Pharmacological and genetic interference with the actin-myosin system abolishes the effect of auxin on vacuoles and thus disrupts its negative influence on cellular growth. SEM-based 3D nanometer-resolution imaging of the vacuoles revealed that auxin controls the constriction and luminal size of the vacuole. We show that this actin-dependent mechanism controls the relative vacuolar occupancy of the cell, thus suggesting an unanticipated mechanism for cytosol homeostasis during cellular growth.
Item Description:First published December 29, 2015
Gesehen am 13.05.2020
Physical Description:Online Resource
ISSN:1091-6490
DOI:10.1073/pnas.1517445113

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