Synthesis of 5′-NAD-capped RNA
In prokaryotic organisms, certain regulatory RNAs have recently been found to be linked to the ubiquitous redox cofactor nicotinamide adenine dinucleotide (NAD) at their 5′-ends. Biochemical and structural investigations of this new caplike RNA modification require synthetic access to pure NAD-RNA....
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
March 4, 2016
|
| In: |
Bioconjugate chemistry
Year: 2016, Volume: 27, Issue: 4, Pages: 874-877 |
| ISSN: | 1520-4812 |
| DOI: | 10.1021/acs.bioconjchem.6b00072 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1021/acs.bioconjchem.6b00072
|
| Author Notes: | Katharina Höfer, Florian Abele, Jasmin Schlotthauer, Andres Jäschke |
| Summary: | In prokaryotic organisms, certain regulatory RNAs have recently been found to be linked to the ubiquitous redox cofactor nicotinamide adenine dinucleotide (NAD) at their 5′-ends. Biochemical and structural investigations of this new caplike RNA modification require synthetic access to pure NAD-RNA. Here we report a chemoenzymatic approach to generate 5′-NAD-capped RNA in high yields and purity under mild conditions. This approach uses unprotected 5′-monophosphate RNA synthesized either chemically or enzymatically, 5′,5′-pyrophosphate bond formation by phosphorimidazolide chemistry, and an enzymatic cleanup step. Thus, 5′-NAD-modified RNA can be synthesized independent of length, structure, and nucleotide sequence. |
|---|---|
| Item Description: | Gesehen am 14.05.2020 |
| Physical Description: | Online Resource |
| ISSN: | 1520-4812 |
| DOI: | 10.1021/acs.bioconjchem.6b00072 |