Synthesis of 5′-NAD-capped RNA
In prokaryotic organisms, certain regulatory RNAs have recently been found to be linked to the ubiquitous redox cofactor nicotinamide adenine dinucleotide (NAD) at their 5′-ends. Biochemical and structural investigations of this new caplike RNA modification require synthetic access to pure NAD-RNA....
Gespeichert in:
| Hauptverfasser: | , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
March 4, 2016
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| In: |
Bioconjugate chemistry
Year: 2016, Jahrgang: 27, Heft: 4, Pages: 874-877 |
| ISSN: | 1520-4812 |
| DOI: | 10.1021/acs.bioconjchem.6b00072 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1021/acs.bioconjchem.6b00072
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| Verfasserangaben: | Katharina Höfer, Florian Abele, Jasmin Schlotthauer, Andres Jäschke |
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| 520 | |a In prokaryotic organisms, certain regulatory RNAs have recently been found to be linked to the ubiquitous redox cofactor nicotinamide adenine dinucleotide (NAD) at their 5′-ends. Biochemical and structural investigations of this new caplike RNA modification require synthetic access to pure NAD-RNA. Here we report a chemoenzymatic approach to generate 5′-NAD-capped RNA in high yields and purity under mild conditions. This approach uses unprotected 5′-monophosphate RNA synthesized either chemically or enzymatically, 5′,5′-pyrophosphate bond formation by phosphorimidazolide chemistry, and an enzymatic cleanup step. Thus, 5′-NAD-modified RNA can be synthesized independent of length, structure, and nucleotide sequence. | ||
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