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MALDI mass spectrometry imaging: a cutting-edge tool for fundamental and clinical histopathology

Histopathological diagnoses have been done in the last century based on hematoxylin and eosin staining. These methods were complemented by histochemistry, electron microscopy, immunohistochemistry (IHC), and molecular techniques. Mass spectrometry (MS) methods allow the thorough examination of vario...

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Main Authors: Longuespée, Rémi (Author) , Casadonte, Rita (Author) , Kriegsmann, Mark (Author) , Pottier, Charles (Author) , Muller, Gaël Picard de (Author) , Delvenne, Philippe (Author) , Kriegsmann, Jörg (Author) , Pauw, Edwin De (Author)
Format: Article (Journal)
Language:English
Published: 17 May 2016
In: Proteomics. Clinical applications
Year: 2016, Volume: 10, Issue: 7, Pages: 701-719
ISSN:1862-8354
DOI:10.1002/prca.201500140
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1002/prca.201500140
Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/prca.201500140
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Author Notes:Rémi Longuespée, Rita Casadonte, Mark Kriegsmann, Charles Pottier, Gaël Picard de Muller, Philippe Delvenne, Jörg Kriegsmann and Edwin De Pauw
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Summary:Histopathological diagnoses have been done in the last century based on hematoxylin and eosin staining. These methods were complemented by histochemistry, electron microscopy, immunohistochemistry (IHC), and molecular techniques. Mass spectrometry (MS) methods allow the thorough examination of various biocompounds in extracts and tissue sections. Today, mass spectrometry imaging (MSI), and especially matrix-assisted laser desorption ionization (MALDI) imaging links classical histology and molecular analyses. Direct mapping is a major advantage of the combination of molecular profiling and imaging. MSI can be considered as a cutting edge approach for molecular detection of proteins, peptides, carbohydrates, lipids, and small molecules in tissues. This review covers the detection of various biomolecules in histopathological sections by MSI. Proteomic methods will be introduced into clinical histopathology within the next few years.
Item Description:Gesehen am 18.05.2020
Physical Description:Online Resource
ISSN:1862-8354
DOI:10.1002/prca.201500140

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