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Gene expression regulatory networks in Trypanosoma brucei: insights into the role of the mRNA-binding proteome

Control of gene expression at the post-transcriptional level is essential in all organisms, and RNA-binding proteins play critical roles from mRNA synthesis to decay. To fully understand this process, it is necessary to identify the complete set of RNA-binding proteins and the functional consequence...

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Bibliographische Detailangaben
Hauptverfasser: Lueong, Smiths (VerfasserIn) , Mercé, Clémentine (VerfasserIn) , Fischer, Bernd (VerfasserIn) , Hoheisel, Jörg D. (VerfasserIn) , Erben, Esteban D. (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 19 January 2016
In: Molecular microbiology
Year: 2016, Jahrgang: 100, Heft: 3, Pages: 457-471
ISSN:1365-2958
DOI:10.1111/mmi.13328
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1111/mmi.13328
Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1111/mmi.13328
Volltext
Verfasserangaben:Smiths Lueong, Clementine Merce, Bernd Fischer, Jörg D. Hoheisel and Esteban D. Erben
Beschreibung
Zusammenfassung:Control of gene expression at the post-transcriptional level is essential in all organisms, and RNA-binding proteins play critical roles from mRNA synthesis to decay. To fully understand this process, it is necessary to identify the complete set of RNA-binding proteins and the functional consequences of the protein-mRNA interactions. Here, we provide an overview of the proteins that bind to mRNAs and their functions in the pathogenic bloodstream form of Trypanosoma brucei. We describe the production of a small collection of open-reading frames encoding proteins potentially involved in mRNA metabolism. With this ORFeome collection, we used tethering to screen for proteins that play a role in post-transcriptional control. A yeast two-hybrid screen showed that several of the discovered repressors interact with components of the CAF1/NOT1 deadenylation complex. To identify the RNA-binding proteins, we obtained the mRNA-bound proteome. We identified 155 high-confidence candidates, including many not previously annotated as RNA-binding proteins. Twenty seven of these proteins affected reporter expression in the tethering screen. Our study provides novel insights into the potential trypanosome mRNPs composition, architecture and function.
Beschreibung:Gesehen am 19.05.2020
Beschreibung:Online Resource
ISSN:1365-2958
DOI:10.1111/mmi.13328

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