Gene expression regulatory networks in Trypanosoma brucei: insights into the role of the mRNA-binding proteome
Control of gene expression at the post-transcriptional level is essential in all organisms, and RNA-binding proteins play critical roles from mRNA synthesis to decay. To fully understand this process, it is necessary to identify the complete set of RNA-binding proteins and the functional consequence...
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| Main Authors: | , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
19 January 2016
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| In: |
Molecular microbiology
Year: 2016, Volume: 100, Issue: 3, Pages: 457-471 |
| ISSN: | 1365-2958 |
| DOI: | 10.1111/mmi.13328 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1111/mmi.13328 Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1111/mmi.13328 
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| Author Notes: | Smiths Lueong, Clementine Merce, Bernd Fischer, Jörg D. Hoheisel and Esteban D. Erben |
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| 520 | |a Control of gene expression at the post-transcriptional level is essential in all organisms, and RNA-binding proteins play critical roles from mRNA synthesis to decay. To fully understand this process, it is necessary to identify the complete set of RNA-binding proteins and the functional consequences of the protein-mRNA interactions. Here, we provide an overview of the proteins that bind to mRNAs and their functions in the pathogenic bloodstream form of Trypanosoma brucei. We describe the production of a small collection of open-reading frames encoding proteins potentially involved in mRNA metabolism. With this ORFeome collection, we used tethering to screen for proteins that play a role in post-transcriptional control. A yeast two-hybrid screen showed that several of the discovered repressors interact with components of the CAF1/NOT1 deadenylation complex. To identify the RNA-binding proteins, we obtained the mRNA-bound proteome. We identified 155 high-confidence candidates, including many not previously annotated as RNA-binding proteins. Twenty seven of these proteins affected reporter expression in the tethering screen. Our study provides novel insights into the potential trypanosome mRNPs composition, architecture and function. | ||
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