Nanoscale alterations of corneocytes indicate skin disease
Background The skin barrier protects the organism against exogenous stressors and simultaneously prevents excessive water loss. While the delicate regulation of skin barrier is not completely understood, morphological and histological evaluation remain key features of clinical investigations. Here,...
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| Main Authors: | , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
2016
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| In: |
Skin research & technology
Year: 2016, Volume: 22, Issue: 2, Pages: 174-180 |
| ISSN: | 1600-0846 |
| DOI: | 10.1111/srt.12247 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1111/srt.12247 Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1111/srt.12247 
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| Author Notes: | J. Franz, M. Beutel, K. Gevers, A. Kramer, J.P. Thyssen, S. Kezic and C. Riethmüller |
| Summary: | Background The skin barrier protects the organism against exogenous stressors and simultaneously prevents excessive water loss. While the delicate regulation of skin barrier is not completely understood, morphological and histological evaluation remain key features of clinical investigations. Here, we extended the phenotypic perspective down to the nanoscale. Methods Corneocyte samples were obtained non-invasively by a standard tape stripping procedure from 21 indviduals. Scanning electron (SEM) and atomic force microcopy (AFM) were used to record nanoscale topography. Circular nano-objects were identified and these were quantitated through computer vision. Results Typical dimensions of 273 nm height and 305 nm width. We showed that their density does not correlate to age or pigmentation in healthy subjects, but that they were clearly elevated in corneocytes from patients with atopic dermatitis, a common inflammatory skin condition. Conclusion The presence of these corneocyte-nanostructures might be used as a diagnostic parameter for skin disorders - even in cases below a clinical threshold. |
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| Item Description: | First published: 22 June 2015 Gesehen am 25.05.2020 |
| Physical Description: | Online Resource |
| ISSN: | 1600-0846 |
| DOI: | 10.1111/srt.12247 |