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Nuclear pores assemble from nucleoporin condensates during oogenesis

The molecular events that direct nuclear pore complex (NPC) assembly toward nuclear envelopes have been conceptualized in two pathways that occur during mitosis or interphase, respectively. In gametes and embryonic cells, NPCs also occur within stacked cytoplasmic membrane sheets, termed annulate la...

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Main Authors: Hampölz, Bernhard (Author) , Schwarz, Andre (Author) , Ronchi, Paolo (Author) , Bragulat-Teixidor, Helena (Author) , Tischer, Christian (Author) , Gaspar, Imre (Author) , Ephrussi, Anne (Author) , Schwab, Yannick (Author) , Beck, Martin (Author)
Format: Article (Journal)
Language:English
Published: 17 October 2019
In: Cell
Year: 2019, Volume: 179, Issue: 3, Pages: 671-686.e17
ISSN:1097-4172
DOI:10.1016/j.cell.2019.09.022
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.cell.2019.09.022
Verlag, lizenzpflichtig, Volltext: http://www.sciencedirect.com/science/article/pii/S0092867419310736
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Author Notes:Bernhard Hampoelz, Andre Schwarz, Paolo Ronchi, Helena Bragulat-Teixidor, Christian Tischer, Imre Gaspar, Anne Ephrussi, Yannick Schwab, and Martin Beck
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Summary:The molecular events that direct nuclear pore complex (NPC) assembly toward nuclear envelopes have been conceptualized in two pathways that occur during mitosis or interphase, respectively. In gametes and embryonic cells, NPCs also occur within stacked cytoplasmic membrane sheets, termed annulate lamellae (AL), which serve as NPC storage for early development. The mechanism of NPC biogenesis at cytoplasmic membranes remains unknown. Here, we show that during Drosophila oogenesis, Nucleoporins condense into different precursor granules that interact and progress into NPCs. Nup358 is a key player that condenses into NPC assembly platforms while its mRNA localizes to their surface in a translation-dependent manner. In concert, Microtubule-dependent transport, the small GTPase Ran and nuclear transport receptors regulate NPC biogenesis in oocytes. We delineate a non-canonical NPC assembly mechanism that relies on Nucleoporin condensates and occurs away from the nucleus under conditions of cell cycle arrest.
Item Description:Gesehen am 28.05.2020
Physical Description:Online Resource
ISSN:1097-4172
DOI:10.1016/j.cell.2019.09.022

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