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Structural characterization of the Chaetomium thermophilum TREX-2 complex and its interaction with the mRNA nuclear export factor Mex67:Mtr2

The TREX-2 complex integrates mRNA nuclear export into the gene expression pathway and is based on a Sac3 scaffold to which Thp1, Sem1, Sus1, and Cdc31 bind. TREX-2 also binds the mRNA nuclear export factor, Mex67:Mtr2, through the Sac3 N-terminal region (Sac3N). Here, we characterize Chaetomium the...

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Hauptverfasser: Dimitrova-Paternoga, Lyudmila Nikolova (VerfasserIn) , Valkov, Eugene (VerfasserIn) , Aibara, Shintaro (VerfasserIn) , Flemming, Dirk (VerfasserIn) , McLaughlin, Stephen H. (VerfasserIn) , Hurt, Ed (VerfasserIn) , Stewart, Murray (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: June 4, 2015
In: Structure
Year: 2015, Jahrgang: 23, Heft: 7, Pages: 1246-1257
ISSN:1878-4186
DOI:10.1016/j.str.2015.05.002
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.str.2015.05.002
Verlag, lizenzpflichtig, Volltext: http://www.sciencedirect.com/science/article/pii/S096921261500180X
Volltext
Verfasserangaben:Lyudmila Dimitrova, Eugene Valkov, Shintaro Aibara, Dirk Flemming, Stephen H. McLaughlin, Ed Hurt, and Murray Stewart
Beschreibung
Zusammenfassung:The TREX-2 complex integrates mRNA nuclear export into the gene expression pathway and is based on a Sac3 scaffold to which Thp1, Sem1, Sus1, and Cdc31 bind. TREX-2 also binds the mRNA nuclear export factor, Mex67:Mtr2, through the Sac3 N-terminal region (Sac3N). Here, we characterize Chaetomium thermophilum TREX-2, show that the in vitro reconstituted complex has an annular structure, and define the structural basis for interactions between Sac3, Sus1, Cdc31, and Mex67:Mtr2. Crystal structures show that the binding of C. thermophilum Sac3N to the Mex67 NTF2-like domain (Mex67NTF2L) is mediated primarily through phenylalanine residues present in a series of repeating sequence motifs that resemble those seen in many nucleoporins, and Mlp1 also binds Mex67:Mtr2 using a similar motif. Deletion of Sac3N generated growth and mRNA export defects in Saccharomyces cerevisiae, and we propose TREX-2 and Mlp1 function to facilitate export by concentrating mature messenger ribonucleoparticles at the nuclear pore entrance.
Beschreibung:Gesehen am 28.05.2020
Beschreibung:Online Resource
ISSN:1878-4186
DOI:10.1016/j.str.2015.05.002

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