An eye on light-sheet microscopy
This chapter introduces the principles and advantages of selective plane illumination microscopy (SPIM) and compares it to commonly used epifluorescence or confocal setups. Due to the low phototoxicity, speed of imaging, high penetration depth, and spatiotemporal resolution, SPIM is predestined for...
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| Main Authors: | , , |
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| Format: | Chapter/Article |
| Language: | English |
| Published: |
27 February 2016
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| In: |
The zebrafish ; part A: Cellular biology
Year: 2016, Pages: 105-123 |
| DOI: | 10.1016/bs.mcb.2016.01.001 |
| Online Access: | Resolving-System, lizenzpflichtig, Volltext: https://doi.org/10.1016/bs.mcb.2016.01.001 Verlag, lizenzpflichtig, Volltext: http://www.sciencedirect.com/science/article/pii/S0091679X16000029 
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| Author Notes: | D. Kromm, T. Thumberger, J. Wittbrodt |
| Summary: | This chapter introduces the principles and advantages of selective plane illumination microscopy (SPIM) and compares it to commonly used epifluorescence or confocal setups. Due to the low phototoxicity, speed of imaging, high penetration depth, and spatiotemporal resolution, SPIM is predestined for in vivo imaging but can as well be used for in toto analysis of large fixed samples. Key points of light-sheet microscopy are highlighted and discussed priming the investigator to choose the best suitable system from the large collection of possible SPIM setups. Mounting of samples is shown and the demands for data acquisition, processing, handling, and visualization are discussed. |
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| Item Description: | Gesehen am 29.05.2020 |
| Physical Description: | Online Resource |
| ISBN: | 0128034890 9780128034897 |
| DOI: | 10.1016/bs.mcb.2016.01.001 |