An eye on light-sheet microscopy
This chapter introduces the principles and advantages of selective plane illumination microscopy (SPIM) and compares it to commonly used epifluorescence or confocal setups. Due to the low phototoxicity, speed of imaging, high penetration depth, and spatiotemporal resolution, SPIM is predestined for...
Gespeichert in:
| Hauptverfasser: | , , |
|---|---|
| Dokumenttyp: | Kapitel/Artikel |
| Sprache: | Englisch |
| Veröffentlicht: |
27 February 2016
|
| In: |
The zebrafish ; part A: Cellular biology
Year: 2016, Pages: 105-123 |
| DOI: | 10.1016/bs.mcb.2016.01.001 |
| Online-Zugang: | Resolving-System, lizenzpflichtig, Volltext: https://doi.org/10.1016/bs.mcb.2016.01.001 Verlag, lizenzpflichtig, Volltext: http://www.sciencedirect.com/science/article/pii/S0091679X16000029 
|
| Verfasserangaben: | D. Kromm, T. Thumberger, J. Wittbrodt |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1699064385 | ||
| 003 | DE-627 | ||
| 005 | 20240215124903.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 200529s2016 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/bs.mcb.2016.01.001 |2 doi | |
| 035 | |a (DE-627)1699064385 | ||
| 035 | |a (DE-599)KXP1699064385 | ||
| 035 | |a (OCoLC)1341326923 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Kromm, Dimitri |d 1988- |e VerfasserIn |0 (DE-588)1209643987 |0 (DE-627)1697329462 |4 aut | |
| 245 | 1 | 3 | |a An eye on light-sheet microscopy |c D. Kromm, T. Thumberger, J. Wittbrodt |
| 264 | 1 | |c 27 February 2016 | |
| 300 | |a 19 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 29.05.2020 | ||
| 520 | |a This chapter introduces the principles and advantages of selective plane illumination microscopy (SPIM) and compares it to commonly used epifluorescence or confocal setups. Due to the low phototoxicity, speed of imaging, high penetration depth, and spatiotemporal resolution, SPIM is predestined for in vivo imaging but can as well be used for in toto analysis of large fixed samples. Key points of light-sheet microscopy are highlighted and discussed priming the investigator to choose the best suitable system from the large collection of possible SPIM setups. Mounting of samples is shown and the demands for data acquisition, processing, handling, and visualization are discussed. | ||
| 650 | 4 | |a DSLM | |
| 650 | 4 | |a Light-sheet | |
| 650 | 4 | |a Medaka | |
| 650 | 4 | |a Mounting | |
| 650 | 4 | |a Selective illumination | |
| 650 | 4 | |a SPIM | |
| 650 | 4 | |a Zebrafish | |
| 700 | 1 | |a Thumberger, Thomas |e VerfasserIn |0 (DE-588)1026677068 |0 (DE-627)727045091 |0 (DE-576)354676075 |4 aut | |
| 700 | 1 | |a Wittbrodt, Joachim |e VerfasserIn |0 (DE-588)1038250919 |0 (DE-627)756835356 |0 (DE-576)167753401 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t The zebrafish ; part A: Cellular biology |b Fourth edition |d Amsterdam : Elsevier, AP, 2016 |g (2016), Seite 105-123 |h 1 Online-Ressource (xvi, 305 Seiten) |w (DE-627)1547687320 |w (DE-576)477687326 |z 0128034890 |z 9780128034897 |7 nnam |a An eye on light-sheet microscopy |
| 773 | 1 | 8 | |g year:2016 |g pages:105-123 |g extent:19 |a An eye on light-sheet microscopy |
| 856 | 4 | 0 | |u https://doi.org/10.1016/bs.mcb.2016.01.001 |x Resolving-System |x Verlag |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u http://www.sciencedirect.com/science/article/pii/S0091679X16000029 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20200529 | ||
| 993 | |a BookComponentPart | ||
| 994 | |a 2016 | ||
| 998 | |g 1038250919 |a Wittbrodt, Joachim |m 1038250919:Wittbrodt, Joachim |d 700000 |d 721000 |e 700000PW1038250919 |e 721000PW1038250919 |k 0/700000/ |k 1/700000/721000/ |p 3 |y j | ||
| 998 | |g 1026677068 |a Thumberger, Thomas |m 1026677068:Thumberger, Thomas |d 700000 |d 721000 |e 700000PT1026677068 |e 721000PT1026677068 |k 0/700000/ |k 1/700000/721000/ |p 2 | ||
| 998 | |g 1209643987 |a Kromm, Dimitri |m 1209643987:Kromm, Dimitri |d 700000 |d 721000 |e 700000PK1209643987 |e 721000PK1209643987 |k 0/700000/ |k 1/700000/721000/ |p 1 |x j | ||
| 999 | |a KXP-PPN1699064385 |e 3675777588 | ||
| BIB | |a Y | ||
| JSO | |a {"name":{"displayForm":["D. Kromm, T. Thumberger, J. Wittbrodt"]},"relHost":[{"physDesc":[{"extent":"1 Online-Ressource (xvi, 305 Seiten)","noteIll":"Illustrationen"}],"id":{"isbn":["0128034890","9780128034897"],"eki":["1547687320"]},"language":["eng"],"disp":"An eye on light-sheet microscopyThe zebrafish ; part A: Cellular biology","origin":[{"dateIssuedDisp":"2016","edition":"Fourth edition","publisher":"Elsevier, AP","editionNo":4,"dateIssuedKey":"2016","publisherPlace":"Amsterdam ; Heidelberg"}],"recId":"1547687320","person":[{"family":"Detrich","display":"Detrich, H. William","roleDisplay":"HerausgeberIn","given":"H. William","role":"edt"},{"role":"edt","given":"Monte","roleDisplay":"HerausgeberIn","family":"Westerfield","display":"Westerfield, Monte"},{"role":"edt","given":"Leonard I.","roleDisplay":"HerausgeberIn","display":"Zon, Leonard I.","family":"Zon"}],"title":[{"title":"Cellular biology / edited by H. William Detrich, III (Northeastern University Marine Science Center, Nahant, MA, United States), Monte Westerfield (University of Oregon, Eugene, OR, United States), Leonard I. Zon (Harvard University, Boston, MA, United States)"}],"part":{"year":"2016","extent":"19","text":"(2016), Seite 105-123","pages":"105-123"},"relMultPart":[{"language":["eng"],"id":{"eki":["1503642089"]},"origin":[{"publisher":"Elsevier/Academic Press","dateIssuedDisp":"20XX-","publisherPlace":"Amsterdam ; Heidelberg [u.a.]"}],"recId":"1503642089","part":{"number":["part A"],"number_sort":["A,4"]},"name":{"displayForm":["ed. by H. William Detrich ..."]},"ga":1,"title":[{"title_sort":"zebrafish","title":"The zebrafish","subtitle":"cellular and developmental biology"}],"person":[{"roleDisplay":"Hrsg.","display":"Detrich, H. William","family":"Detrich","given":"H. William","role":"edt"}],"type":{"media":"Online-Ressource","bibl":"edited-book"}},{"ga":1,"part":{"number_sort":["133"],"number":["volume 133"]},"origin":[{"publisher":"Elsevier","dateIssuedDisp":"2004-","publisherPlace":"New York, NY [u.a.]","dateIssuedKey":"2004"}],"pubHistory":["Nachgewiesen 76.2004 -"],"disp":"Methods in cell biology","type":{"media":"Online-Ressource","bibl":"serial"},"dispAlt":"Methods in cell biology","title":[{"title":"Methods in cell biology","title_sort":"Methods in cell biology"}],"recId":"520196031","id":{"eki":["520196031"],"zdb":["2257731-2"],"issn":["0091-679X"]},"language":["eng"],"physDesc":[{"extent":"Online-Ressource"}]}],"type":{"bibl":"book","media":"Online-Ressource"}}],"origin":[{"dateIssuedDisp":"27 February 2016","dateIssuedKey":"2016"}],"type":{"bibl":"chapter","media":"Online-Ressource"},"note":["Gesehen am 29.05.2020"],"title":[{"title_sort":"eye on light-sheet microscopy","title":"An eye on light-sheet microscopy"}],"person":[{"role":"aut","given":"Dimitri","display":"Kromm, Dimitri","family":"Kromm","roleDisplay":"VerfasserIn"},{"roleDisplay":"VerfasserIn","display":"Thumberger, Thomas","family":"Thumberger","given":"Thomas","role":"aut"},{"given":"Joachim","role":"aut","roleDisplay":"VerfasserIn","family":"Wittbrodt","display":"Wittbrodt, Joachim"}],"recId":"1699064385","language":["eng"],"id":{"eki":["1699064385"],"doi":["10.1016/bs.mcb.2016.01.001"]},"physDesc":[{"extent":"19 S."}]} | ||
| SRT | |a KROMMDIMITEYEONLIGHT2720 | ||