Nanopore diameters tune strain in extruded fibronectin fibers
Fibronectin is present in the extracellular matrix and can be assembled into nanofibers in vivo by undergoing conformational changes. Here, we present a novel approach to prepare fibronectin nanofibers under physiological conditions using an extrusion approach through nanoporous aluminum oxide membr...
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| Autori principali: | , , , , , |
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| Natura: | Article (Journal) |
| Lingua: | inglese |
| Pubblicazione: |
September 11, 2015
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| In: |
Nano letters
Year: 2015, Volume: 15, Fascicolo: 10, Pages: 6357-6364 |
| ISSN: | 1530-6992 |
| DOI: | 10.1021/acs.nanolett.5b01356 |
| Accesso online: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1021/acs.nanolett.5b01356
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| Note sull'autore: | Mohammad Raoufi, Tamal Das, Ingmar Schoen, Viola Vogel, Dorothea Brüggemann, and Joachim P. Spatz |
| Riassunto: | Fibronectin is present in the extracellular matrix and can be assembled into nanofibers in vivo by undergoing conformational changes. Here, we present a novel approach to prepare fibronectin nanofibers under physiological conditions using an extrusion approach through nanoporous aluminum oxide membranes. This one-step process can prepare nanofiber bundles up to a millimeter in length and with uniform fiber diameters in the nanometer range. Most importantly, by using different pore diameters and protein concentrations in the extrusion process, we could induce varying lasting structural changes in the fibers, which were monitored by Förster resonance energy transfer and should impose different physiological functions. |
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| Descrizione del documento: | Gesehen am 10.06.2020 |
| Descrizione fisica: | Online Resource |
| ISSN: | 1530-6992 |
| DOI: | 10.1021/acs.nanolett.5b01356 |