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2D map projections for visualization and quantitative analysis of 3D fluorescence micrographs

We introduce Map3-2D, a freely available software to accurately project up to five-dimensional (5D) fluorescence microscopy image data onto full-content 2D maps. Similar to the Earth’s projection onto cartographic maps, Map3-2D unfolds surface information from a stack of images onto a single, struct...

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Bibliographic Details
Main Authors: Sendra, Hernán Gonzalo (Author) , Hoerth, Christian (Author) , Wunder, Christian (Author) , Lorenz, Holger (Author)
Format: Article (Journal)
Language:English
Published: 24 July 2015
In: Scientific reports
Year: 2015, Volume: 5
ISSN:2045-2322
DOI:10.1038/srep12457
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/srep12457
Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/srep12457
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Author Notes:G. Hernán Sendra, Christian H. Hoerth, Christian Wunder & Holger Lorenz
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Summary:We introduce Map3-2D, a freely available software to accurately project up to five-dimensional (5D) fluorescence microscopy image data onto full-content 2D maps. Similar to the Earth’s projection onto cartographic maps, Map3-2D unfolds surface information from a stack of images onto a single, structurally connected map. We demonstrate its applicability for visualization and quantitative analyses of spherical and uneven surfaces in fixed and dynamic live samples by using mammalian and yeast cells and giant unilamellar vesicles. Map3-2D software is available at http://www.zmbh.uni-heidelberg.de//Central_Services/Imaging_Facility/Map3-2D.html.
Item Description:Gesehen am 16.06.2020
Physical Description:Online Resource
ISSN:2045-2322
DOI:10.1038/srep12457

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