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Nanoscopic localization of surface-exposed antigens of Borrelia burgdorferi

Borrelia burgdorferi sensu lato, the causative agent of Lyme disease, is transmitted to humans through the bite of infected Ixodes spp. ticks. Successful infection of vertebrate hosts necessitates sophisticated means of the pathogen to escape the vertebrates’ immune system. One strategy employed by...

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Main Authors: Lemgruber, Leandro (Author) , Sant’Anna, Celso (Author) , Griffths, Caron (Author) , Abud, Yuri (Author) , Mhlanga, Musa (Author) , Wallich, Reinhard (Author) , Frischknecht, Friedrich (Author)
Format: Article (Journal)
Language:English
Published: 05 March 2015
In: Microscopy and microanalysis
Year: 2015, Volume: 21, Issue: 3, Pages: 680-688
ISSN:1435-8115
DOI:10.1017/S1431927615000318
Online Access:Resolving-System, lizenzpflichtig, Volltext: https://doi.org/10.1017/S1431927615000318
Verlag, lizenzpflichtig, Volltext: https://www.cambridge.org/core/journals/microscopy-and-microanalysis/article/nanoscopic-localization-of-surfaceexposed-antigens-of-borrelia-burgdorferi/200B00A6FCC637901AAD62259D19C942
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Author Notes:Leandro Lemgruber, Celso Sant’Anna, Caron Griffths, Yuri Abud, Musa Mhlanga, Reinhard Wallich, and Friedrich Frischknecht
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Summary:Borrelia burgdorferi sensu lato, the causative agent of Lyme disease, is transmitted to humans through the bite of infected Ixodes spp. ticks. Successful infection of vertebrate hosts necessitates sophisticated means of the pathogen to escape the vertebrates’ immune system. One strategy employed by Lyme disease spirochetes to evade adaptive immunity involves a highly coordinated regulation of the expression of outer surface proteins that is vital for infection, dissemination, and persistence. Here we characterized the expression pattern of bacterial surface antigens using different microscopy techniques, from fluorescent wide field to super-resolution and immunogold-scanning electron microscopy. A fluorescent strain of B. burgdorferi spirochetes was labeled with monoclonal antibodies directed against various bacterial surface antigens. Our results indicate that OspA is more evenly distributed over the surface than OspB and OspC that were present as punctate areas.
Item Description:Gesehen am 17.06.2020
Physical Description:Online Resource
ISSN:1435-8115
DOI:10.1017/S1431927615000318

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