Poly-acrylic acid brushes and adsorbed proteins
<section class="abstract"><h2 class="abstractTitle text-title my-1" id="d635e2">Abstract</h2><p>Planar polyelectrolyte brushes are prepared by Langmuir-Schaefer - based grafting of perdeuterated (styrene)<sub>49</sub>-b-(acrylic - aci...
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| Main Authors: | , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
02.04.2015
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| In: |
Zeitschrift für physikalische Chemie
Year: 2015, Volume: 229, Issue: 7-8, Pages: 1119-1139 |
| ISSN: | 2196-7156 |
| DOI: | 10.1515/zpch-2014-0540 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1515/zpch-2014-0540 Verlag, lizenzpflichtig, Volltext: https://www.degruyterbrill.com/view/journals/zpch/229/7-8/article-p1119.xml 
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| Author Notes: | Matthias Reinhardt, Martin Kreuzer, Thomas Geue, Reiner Dahint, Matthias Ballauff, Roland Steitz |
| Summary: | <section class="abstract"><h2 class="abstractTitle text-title my-1" id="d635e2">Abstract</h2><p>Planar polyelectrolyte brushes are prepared by Langmuir-Schaefer - based grafting of perdeuterated (styrene)<sub>49</sub>-b-(acrylic - acid)<sub>222</sub> block copolymer (dPS-b-PAA) to dPS pre-coated silicon - supports with grafting density <em>σ</em><sub>PAA</sub> from 0.07 to - 0.11 nm<sup><em>-</em>2</sup>. The structure of the solvent-swollen brushes, - i. e. the volume fraction profile of polymer segments, <em>ϕ</em><sub>PAA</sub>, as a function of altitude <em>z</em> from the grafting - plane into the liquid phase is extracted from neutron reflectivity - measurements. We find that for all cases investigated - <em>ϕ</em><sub>PAA</sub>(<em>z</em>) resembles a Gaussian profile. Although very - condensed, the PAA brushes can be loaded with bovine serum albumin - (BSA). The integral amount of adsorbed BSA scales linearly with - grafting density. We compare our <em>z</em>-resolved volume fraction profile - <em>ϕ</em><sub>BSA</sub>(<em>z</em>) of BSA on PAA brushes with existing literature - on that system. It is found that a cross-over takes place in the - adsorption scheme from ternary compressive, where proteins can - approach the grafting surface only by compressing the brush, to - ternary insertive, where proteins enter the brush with only local - perturbation of the concentration profile, as a function of - <em>R</em><sub>P</sub>/<em>H</em><sub>max</sub>, where <em>R</em><sub>P</sub> is the - Stokes-Radius of the protein, and <em>H</em><sub>max</sub> is the - experimentally determined maximum height of - the brush.</p></section> |
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| Item Description: | Gesehen am 17.06.2020 |
| Physical Description: | Online Resource |
| ISSN: | 2196-7156 |
| DOI: | 10.1515/zpch-2014-0540 |