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Light-induced protein dimerization by one- and two-photon activation of gibberellic acid derivatives in living cells

We developed a highly efficient system for light-induced protein dimerization in live cells using photo-caged derivatives of the phytohormone gibberellic acid (GA3). We demonstrate the application of the photo-activatable chemical inducer of dimerization (CID) for the control of protein translocatio...

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Main Authors: Schelkle, Korwin (Author) , Griesbaum, Tristan (Author) , Ollech, Dirk (Author) , Becht, Steffy (Author) , Buckup, Tiago (Author) , Hamburger, Manuel (Author) , Wombacher, Richard (Author)
Format: Article (Journal)
Language:English
Published: January 13, 2015
In: Angewandte Chemie. International edition
Year: 2015, Volume: 54, Issue: 9, Pages: 2825-2829
ISSN:1521-3773
DOI:10.1002/anie.201409196
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1002/anie.201409196
Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/anie.201409196
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Author Notes:Korwin M. Schelkle, Tristan Griesbaum, Dirk Ollech, Steffy Becht, Tiago Buckup, Manuel Hamburger, and Richard Wombacher
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Summary:We developed a highly efficient system for light-induced protein dimerization in live cells using photo-caged derivatives of the phytohormone gibberellic acid (GA3). We demonstrate the application of the photo-activatable chemical inducer of dimerization (CID) for the control of protein translocation with high spatiotemporal precision using light as an external trigger. Furthermore, we present a new two-photon (2P)-sensitive caging group, whose exceptionally high two-photon cross section allows the use of infrared light to efficiently unleash the active GA3 for inducing protein dimerization in living cells.
Item Description:Gesehen am 18.06.2020
Physical Description:Online Resource
ISSN:1521-3773
DOI:10.1002/anie.201409196

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