Heparin modulates chemokines in human endometrial stromal cells by interaction with tumor necrosis factor α and thrombin
Objective - To study the impact of heparins on chemokines in decidualized human endometrial stromal cells (ESCs) in vitro. - Design - In vitro experiment. - Setting - Research laboratory. - Patient(s) - Premenopausal women undergoing hysterectomy for benign reasons. - Intervention(s) - ESCs were iso...
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| Main Authors: | , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
March 23, 2015
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| In: |
Fertility and sterility
Year: 2015, Volume: 103, Issue: 5, Pages: 1363-1369 |
| ISSN: | 1556-5653 |
| DOI: | 10.1016/j.fertnstert.2015.02.023 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.fertnstert.2015.02.023 Verlag, lizenzpflichtig, Volltext: http://www.sciencedirect.com/science/article/pii/S0015028215001533 
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| Author Notes: | Julia Spratte M.D., Magdalena Schönborn B.S., Nora Treder B.S., Frauke Bornkessel B.S., Marek Zygmunt M.D., Ph.D., Herbert Fluhr M.D., Ph.D. |
| Summary: | Objective - To study the impact of heparins on chemokines in decidualized human endometrial stromal cells (ESCs) in vitro. - Design - In vitro experiment. - Setting - Research laboratory. - Patient(s) - Premenopausal women undergoing hysterectomy for benign reasons. - Intervention(s) - ESCs were isolated from hysterectomy specimens, decidualized in vitro and incubated with unfractionated heparin and low-molecular-weight heparins (LMWHs) as well as tumor necrosis factor (TNF) α or thrombin with or without heparins. - Main Outcome Measure(s) - Chemokines CXCL1, CXCL5, CXCL8, CCL2, and CCL5 were measured with the use of ELISA, and CXCL5, CXCL8, CCL2, and CCL5 were detected with the use of real-time reverse-transcription polymerase chain reaction. Cell viability was determined with the use of a fluorometric assay. - Result(s) - TNF-α and thrombin stimulated distinct patterns of chemokines in ESCs. Unfractionated heparin and LMWHs attenuated the TNF-α-mediated induction of CXCL8 and enhanced CXCL5, CCL2, and CCL5. The stimulating effect of thrombin on CXCL8 could be inhibited by heparin, whereas heparin had no impact on thrombin-induced CXCL1 and CCL2. Nuclear factor of transcription κB signaling mediated the effects of TNF-α. The effects of thrombin were mediated via extracellular signal-regulated protein kinases 1/2. - Conclusion(s) - Heparins have modulating effects on TNF-α- and thrombin-induced endometrial chemokines, which might have implications in the regulation of endometrial receptivity and early implantation. |
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| Item Description: | Gesehen am 22.06.2020 |
| Physical Description: | Online Resource |
| ISSN: | 1556-5653 |
| DOI: | 10.1016/j.fertnstert.2015.02.023 |