Freely drawn single lipid nanotube patterns
LNTs are unique 3D structures made only of safe and abundant biomaterials by self-assembly. The current bottleneck for developing applications using LNTs is the lack of an easy technique to pattern them on substrates. We report a method to free-draw single lipid nanotube (LNT) patterns in any shape...
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| Main Authors: | , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
20 Jan 2015
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| In: |
Soft matter
Year: 2015, Volume: 11, Issue: 10, Pages: 2029-2035 |
| ISSN: | 1744-6848 |
| DOI: | 10.1039/C5SM00043B |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1039/C5SM00043B Verlag, lizenzpflichtig, Volltext: https://pubs.rsc.org/en/content/articlelanding/2015/sm/c5sm00043b 
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| Author Notes: | Kaori Sugihara, Amin Rustom and Joachim P. Spatz |
| Summary: | LNTs are unique 3D structures made only of safe and abundant biomaterials by self-assembly. The current bottleneck for developing applications using LNTs is the lack of an easy technique to pattern them on substrates. We report a method to free-draw single lipid nanotube (LNT) patterns in any shape on surfaces with 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) that takes an inverted hexagonal (HII) phase. We used pre-self-assembled LNTs or HII lipid blocks as a lipid reservoir from which new LNTs were pulled by applying a point load with a micromanipulator. The extreme simplicity of our technique originates from the fundamental nature of DOPE lipids that prefer a HII phase, while all the conventional approaches use PC lipids that form a lamellar phase. By adjusting the surface properties with polyelectrolyte multilayers, the created single LNT objects are able to remain adhered to the surface for over a week. Importantly, it could be shown that two vesicles loaded with caged fluorescent molecules were able to fuse well with a LNT, enabling diffusive transport of uncaged fluorescent molecules from one vesicle to the other. |
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| Item Description: | Gesehen am 23.06.2020 |
| Physical Description: | Online Resource |
| ISSN: | 1744-6848 |
| DOI: | 10.1039/C5SM00043B |