Generation of a synthetic GlcNAcylated nucleosome reveals regulation of stability by H2A-Thr101 GlcNAcylation
O-GlcNAcylation is a newly discovered histone modification implicated in transcriptional regulation, but no structural information on the physical effect of GlcNAcylation on chromatin exists. Here, we generate synthetic, pure GlcNAcylated histones and nucleosomes and reveal that GlcNAcylation can mo...
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| Main Authors: | , , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
25 Aug 2015
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| In: |
Nature Communications
Year: 2015, Volume: 6, Issue: 1 |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/ncomms8978 |
| Online Access: | Resolving-System, lizenzpflichtig, Volltext: https://doi.org/10.1038/ncomms8978 Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/ncomms8978 
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| Author Notes: | Lukas Lercher, Ritu Raj, Nisha A. Patel, Joshua Price, Shabaz Mohammed, Carol V. Robinson, Christopher J. Schofield & Benjamin G. Davis |
| Summary: | O-GlcNAcylation is a newly discovered histone modification implicated in transcriptional regulation, but no structural information on the physical effect of GlcNAcylation on chromatin exists. Here, we generate synthetic, pure GlcNAcylated histones and nucleosomes and reveal that GlcNAcylation can modulate structure through direct destabilization of H2A/H2B dimers in the nucleosome, thus promoting an ‘open’ chromatin state. The results suggest that a plausible molecular basis for one role of histone O-GlcNAcylation in epigenetic regulation is to lower the barrier for RNA polymerase passage and hence increase transcription. |
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| Item Description: | Gesehen am 26.06.2020 |
| Physical Description: | Online Resource |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/ncomms8978 |