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Monitoring of short-term erythropoietin therapy in rats with acute spinal cord injury using manganese-enhanced Magnetic Resonance Imaging

BACKGROUND AND PURPOSE To evaluate the short-term outcome of erythropoietin (EPO) therapy in rats with spinal cord injury (SCI) using manganese-enhanced magnetic resonance imaging (MEMRI). METHODS Rats were divided in an EPO and a control group. Laminectomy at Th11 was performed, followed by SCI. Mn...

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Hauptverfasser: Freitag, Martin T. (VerfasserIn) , Parzer, Peter (VerfasserIn) , Stieltjes, Bram (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 2015
In: Journal of neuroimaging
Year: 2014, Jahrgang: 25, Heft: 4, Pages: 582-589
ISSN:1552-6569
DOI:10.1111/jon.12202
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1111/jon.12202
Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1111/jon.12202
Volltext
Verfasserangaben:Martin Thomas Freitag, MD, Gábor Márton, MSc, Krisztián Pajer, MSc, Jens Hartmann, MD, Nadja Walder, MD, Markus Rossmann, MD, Peter Parzer, MSc, Heinz Redl, PhD, Antal Nógrádi, MD, PhD, DSc*, Bram Stieltjes, MD, PhD*
Beschreibung
Zusammenfassung:BACKGROUND AND PURPOSE To evaluate the short-term outcome of erythropoietin (EPO) therapy in rats with spinal cord injury (SCI) using manganese-enhanced magnetic resonance imaging (MEMRI). METHODS Rats were divided in an EPO and a control group. Laminectomy at Th11 was performed, followed by SCI. MnCl2 was applied into the cisterna magna and functional recovery was examined after injury using BBB-scoring. Then, rats were euthanized and the spinal cord was extracted for MEMRI. Finally, histological analysis was performed and correlated with MEMRI. RESULTS EPO-treated animals showed significantly better functional recovery (P = .008, r = .62) and higher mean signal-to-noise ratio (SNR) in MEMRI compared to controls for slices 10-13 (P = .017, R2 = .31) at the level of the lesion epicenter. Functional recovery correlated significantly with higher SNR values, determined using the mean SNR between slices 10 and 13 (P = .047, R2 = .36). In this region, histology revealed a significantly decreased number of microglia cells and apoptosis in EPO-treated animals. CONCLUSION MEMRI successfully depicts the therapeutic effect of EPO in early SCI that leads to a significant recovery in rats, a significantly reduced immune response and significantly reduced number of apoptotic cells at the height of the lesion epicenter.
Beschreibung:First published: 16 December 2014
Gesehen am 26.06.20
Beschreibung:Online Resource
ISSN:1552-6569
DOI:10.1111/jon.12202

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