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Response to comment on “global diversity and geography of soil fungi”

Schadt and Rosling (Technical Comment, 26 June 2015, p. 1438) argue that primer-template mismatches neglected the fungal class Archaeorhizomycetes in a global soil survey. Amplicon-based metabarcoding of nine barcode-primer pair combinations and polymerase chain reaction (PCR)-free shotgun metagenom...

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Main Authors: Tedersoo, Leho (Author) , Bahram, Mohammad (Author) , Põlme, Sergei (Author) , Anslan, Sten (Author) , Riit, Taavi (Author) , Kõljalg, Urmas (Author) , Nilsson, R. Henrik (Author) , Hildebrand, Falk (Author) , Abarenkov, Kessy (Author)
Format: Article (Journal)
Language:English
Published: 28 Aug 2015
In: Science
Year: 2015, Volume: 349, Issue: 6251, Pages: 936d-936c
ISSN:1095-9203
DOI:10.1126/science.aaa5594
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1126/science.aaa5594
Verlag, lizenzpflichtig, Volltext: https://science.sciencemag.org/content/349/6251/936.4
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Author Notes:Leho Tedersoo, Mohammad Bahram, Sergei Põlme, Sten Anslan, Taavi Riit, Urmas Kõljalg, R. Henrik Nilsson, Falk Hildebrand, Kessy Abarenkov
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Summary:Schadt and Rosling (Technical Comment, 26 June 2015, p. 1438) argue that primer-template mismatches neglected the fungal class Archaeorhizomycetes in a global soil survey. Amplicon-based metabarcoding of nine barcode-primer pair combinations and polymerase chain reaction (PCR)-free shotgun metagenomics revealed that barcode and primer choice and PCR bias drive the diversity and composition of microorganisms in general, but the Archaeorhizomycetes were little affected in the global study. We urge that careful choice of DNA markers and primers is essential for ecological studies using high-throughput sequencing for identification.
Item Description:Gesehen am 30.06.2020
Physical Description:Online Resource
ISSN:1095-9203
DOI:10.1126/science.aaa5594

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