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Nanofocused scanning X-ray fluorescence microscopy revealing an effect of heterozygous hemoglobin S and C on biochemical activities in plasmodium falciparum-infected erythrocytes

While there is ample evidence suggesting that carriers of heterozygous hemoglobin S and C are protected from life- threatening malaria, little is known about the underlying biochemical mechanisms at the single cell level. Using nanofocused scanning X-ray fluorescence microscopy, we quantify the spat...

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Bibliographic Details
Main Authors: Fröhlich, Benjamin (Author) , Yang, Yang (Author) , Thoma, Judith (Author) , Czajor, Julian (Author) , Lansche, Christine (Author) , Sanchez, Cecilia P. (Author) , Lanzer, Michael (Author) , Cloetens, Peter (Author) , Tanaka, Motomu (Author)
Format: Article (Journal)
Language:English
Published: March 23, 2020
In: Analytical chemistry
Year: 2020, Volume: 92, Issue: 8, Pages: 5765-5771
ISSN:1520-6882
DOI:10.1021/acs.analchem.9b05111
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1021/acs.analchem.9b05111
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Author Notes:Benjamin Fröhlich, Yang Yang, Judith Thoma, Julian Czajor, Christine Lansche, Cecilia Sanchez, Michael Lanzer, Peter Cloetens, and Motomu Tanaka
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Summary:While there is ample evidence suggesting that carriers of heterozygous hemoglobin S and C are protected from life- threatening malaria, little is known about the underlying biochemical mechanisms at the single cell level. Using nanofocused scanning X-ray fluorescence microscopy, we quantify the spatial distribution of individual elements in subcellular compartments, including Fe, S, P, Zn, and Cu, in Plasmodium falciparum-infected (P. falciparum-infected) erythrocytes carrying the wild type or variant hemoglobins. Our data indicate that heterozygous hemoglobin S and C significantly modulate biochemical reactions in parasitized erythrocytes, such as aberrant hemozoin mineralization and a delay in hemoglobin degradation. The label-free scanning X-ray fluorescence imaging has great potential to quantify the spatial distribution of elements in subcellular compartments of P. falciparum-infected erythrocytes and unravel the biochemical mechanisms underpinning disease and protective traits.
Item Description:Gesehen am 02.07.2020
Physical Description:Online Resource
ISSN:1520-6882
DOI:10.1021/acs.analchem.9b05111

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