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Nanofocused scanning X-ray fluorescence microscopy revealing an effect of heterozygous hemoglobin S and C on biochemical activities in plasmodium falciparum-infected erythrocytes

While there is ample evidence suggesting that carriers of heterozygous hemoglobin S and C are protected from life- threatening malaria, little is known about the underlying biochemical mechanisms at the single cell level. Using nanofocused scanning X-ray fluorescence microscopy, we quantify the spat...

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Hauptverfasser: Fröhlich, Benjamin (VerfasserIn) , Yang, Yang (VerfasserIn) , Thoma, Judith (VerfasserIn) , Czajor, Julian (VerfasserIn) , Lansche, Christine (VerfasserIn) , Sanchez, Cecilia P. (VerfasserIn) , Lanzer, Michael (VerfasserIn) , Cloetens, Peter (VerfasserIn) , Tanaka, Motomu (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: March 23, 2020
In: Analytical chemistry
Year: 2020, Jahrgang: 92, Heft: 8, Pages: 5765-5771
ISSN:1520-6882
DOI:10.1021/acs.analchem.9b05111
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1021/acs.analchem.9b05111
Volltext
Verfasserangaben:Benjamin Fröhlich, Yang Yang, Judith Thoma, Julian Czajor, Christine Lansche, Cecilia Sanchez, Michael Lanzer, Peter Cloetens, and Motomu Tanaka
Beschreibung
Zusammenfassung:While there is ample evidence suggesting that carriers of heterozygous hemoglobin S and C are protected from life- threatening malaria, little is known about the underlying biochemical mechanisms at the single cell level. Using nanofocused scanning X-ray fluorescence microscopy, we quantify the spatial distribution of individual elements in subcellular compartments, including Fe, S, P, Zn, and Cu, in Plasmodium falciparum-infected (P. falciparum-infected) erythrocytes carrying the wild type or variant hemoglobins. Our data indicate that heterozygous hemoglobin S and C significantly modulate biochemical reactions in parasitized erythrocytes, such as aberrant hemozoin mineralization and a delay in hemoglobin degradation. The label-free scanning X-ray fluorescence imaging has great potential to quantify the spatial distribution of elements in subcellular compartments of P. falciparum-infected erythrocytes and unravel the biochemical mechanisms underpinning disease and protective traits.
Beschreibung:Gesehen am 02.07.2020
Beschreibung:Online Resource
ISSN:1520-6882
DOI:10.1021/acs.analchem.9b05111

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