Nanofocused scanning X-ray fluorescence microscopy revealing an effect of heterozygous hemoglobin S and C on biochemical activities in plasmodium falciparum-infected erythrocytes
While there is ample evidence suggesting that carriers of heterozygous hemoglobin S and C are protected from life- threatening malaria, little is known about the underlying biochemical mechanisms at the single cell level. Using nanofocused scanning X-ray fluorescence microscopy, we quantify the spat...
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| Main Authors: | , , , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
March 23, 2020
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| In: |
Analytical chemistry
Year: 2020, Volume: 92, Issue: 8, Pages: 5765-5771 |
| ISSN: | 1520-6882 |
| DOI: | 10.1021/acs.analchem.9b05111 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1021/acs.analchem.9b05111
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| Author Notes: | Benjamin Fröhlich, Yang Yang, Judith Thoma, Julian Czajor, Christine Lansche, Cecilia Sanchez, Michael Lanzer, Peter Cloetens, and Motomu Tanaka |
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| 520 | |a While there is ample evidence suggesting that carriers of heterozygous hemoglobin S and C are protected from life- threatening malaria, little is known about the underlying biochemical mechanisms at the single cell level. Using nanofocused scanning X-ray fluorescence microscopy, we quantify the spatial distribution of individual elements in subcellular compartments, including Fe, S, P, Zn, and Cu, in Plasmodium falciparum-infected (P. falciparum-infected) erythrocytes carrying the wild type or variant hemoglobins. Our data indicate that heterozygous hemoglobin S and C significantly modulate biochemical reactions in parasitized erythrocytes, such as aberrant hemozoin mineralization and a delay in hemoglobin degradation. The label-free scanning X-ray fluorescence imaging has great potential to quantify the spatial distribution of elements in subcellular compartments of P. falciparum-infected erythrocytes and unravel the biochemical mechanisms underpinning disease and protective traits. | ||
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