Viral infection at high magnification: 3D electron microscopy methods to analyze the architecture of infected cells
As obligate intracellular parasites, viruses need to hijack their cellular hosts and reprogram their machineries in order to replicate their genomes and produce new virions. For the direct visualization of the different steps of a viral life cycle (attachment, entry, replication, assembly and egress...
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| Main Authors: | , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
3 December 2015
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| In: |
Viruses
Year: 2015, Volume: 7, Issue: 12, Pages: 6316-6345 |
| ISSN: | 1999-4915 |
| DOI: | 10.3390/v7122940 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.3390/v7122940 Verlag, lizenzpflichtig, Volltext: https://www.mdpi.com/1999-4915/7/12/2940 
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| Author Notes: | Inés Romero-Brey and Ralf Bartenschlager |
| Summary: | As obligate intracellular parasites, viruses need to hijack their cellular hosts and reprogram their machineries in order to replicate their genomes and produce new virions. For the direct visualization of the different steps of a viral life cycle (attachment, entry, replication, assembly and egress) electron microscopy (EM) methods are extremely helpful. While conventional EM has given important information about virus-host cell interactions, the development of three-dimensional EM (3D-EM) approaches provides unprecedented insights into how viruses remodel the intracellular architecture of the host cell. During the last years several 3D-EM methods have been developed. Here we will provide a description of the main approaches and examples of innovative applications. |
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| Item Description: | Gesehen am 02.07.2020 |
| Physical Description: | Online Resource |
| ISSN: | 1999-4915 |
| DOI: | 10.3390/v7122940 |