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Polysomes of Trypanosoma brucei: association with initiation factors and RNA-binding proteins

We report here the results of experiments designed to identify RNA-binding proteins that might be associated with Trypanosoma brucei polysomes. After some preliminary mass spectrometry of polysomal fractions, we investigated the distributions of selected tagged proteins using sucrose gradients and i...

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Bibliographic Details
Main Authors: Klein, Cornelia Andrea (Author) , Terrao, Monica (Author) , Inchaustegui Gil, Diana Patricia (Author) , Clayton, Christine (Author)
Format: Article (Journal)
Language:English
Published: August 19, 2015
In: PLOS ONE
Year: 2015, Volume: 10, Issue: 8
ISSN:1932-6203
DOI:10.1371/journal.pone.0135973
Online Access:Resolving-System, lizenzpflichtig, Volltext: https://doi.org/10.1371/journal.pone.0135973
Verlag, lizenzpflichtig, Volltext: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0135973
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Author Notes:Cornelia Klein, Monica Terrao, Diana Inchaustegui Gil, Christine Clayton
Description
Summary:We report here the results of experiments designed to identify RNA-binding proteins that might be associated with Trypanosoma brucei polysomes. After some preliminary mass spectrometry of polysomal fractions, we investigated the distributions of selected tagged proteins using sucrose gradients and immunofluorescence.
Item Description:Gesehen am 07.07.2020
Physical Description:Online Resource
ISSN:1932-6203
DOI:10.1371/journal.pone.0135973

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