Cover Image

Three gangliogliomas: results of GTG-banding, SKY, genome-wide high resolution SNP-array, gene expression and review of the literature

According to the World Health Organization gangliogliomas are classified as well-differentiated and slowly growing neuroepithelial tumors, composed of neoplastic mature ganglion and glial cells. It is the most frequent tumor entity observed in patients with long-term epilepsy. Comprehensive cytogene...

Full description

Saved in:
Bibliographic Details
Main Authors: Xu, Li-Xin (Author) , Holland, Heidrun (Author) , Kirsten, Holger (Author) , Ahnert, Peter (Author) , Krupp, Wolfgang (Author) , Bauer, Manfred (Author) , Schober, Ralf (Author) , Mueller, Wolf (Author) , Fritzsch, Dominik (Author) , Meixensberger, Jürgen (Author) , Koschny, Ronald (Author)
Format: Article (Journal)
Language:English
Published: 2015
In: Neuropathology
Year: 2015, Volume: 35, Issue: 2, Pages: 148-157
ISSN:1440-1789
DOI:10.1111/neup.12176
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1111/neup.12176
Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1111/neup.12176
Get full text
Author Notes:Li-Xin Xu, Heidrun Holland, Holger Kirsten, Peter Ahnert, Wolfgang Krupp, Manfred Bauer, Ralf Schober, Wolf Mueller, Dominik Fritzsch, Jürgen Meixensberger and Ronald Koschny
Description
Summary:According to the World Health Organization gangliogliomas are classified as well-differentiated and slowly growing neuroepithelial tumors, composed of neoplastic mature ganglion and glial cells. It is the most frequent tumor entity observed in patients with long-term epilepsy. Comprehensive cytogenetic and molecular cytogenetic data including high-resolution genomic profiling (single nucleotide polymorphism (SNP)-array) of gangliogliomas are scarce but necessary for a better oncological understanding of this tumor entity. For a detailed characterization at the single cell and cell population levels, we analyzed genomic alterations of three gangliogliomas using trypsin-Giemsa banding (GTG-banding) and by spectral karyotyping (SKY) in combination with SNP-array and gene expression array experiments. By GTG and SKY, we could confirm frequently detected chromosomal aberrations (losses within chromosomes 10, 13 and 22; gains within chromosomes 5, 7, 8 and 12), and identify so far unknown genetic aberrations like the unbalanced non-reciprocal translocation t(1;18)(q21;q21). Interestingly, we report on the second so far detected ganglioglioma with ring chromosome 1. Analyses of SNP-array data from two of the tumors and respective germline DNA (peripheral blood) identified few small gains and losses and a number of copy-neutral regions with loss of heterozygosity (LOH) in germline and in tumor tissue. In comparison to germline DNA, tumor tissues did not show substantial regions with significant loss or gain or with newly developed LOH. Gene expression analyses of tumor-specific genes revealed similarities in the profile of the analyzed samples regarding different relevant pathways. Taken together, we describe overlapping but also distinct and novel genetic aberrations of three gangliogliomas.
Item Description:Gesehen am 07.07.2020
First published: 06 November 2014
Physical Description:Online Resource
ISSN:1440-1789
DOI:10.1111/neup.12176

Similar Items