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In vitro maturation of oocytes is not associated with altered deoxyribonucleic acid methylation patterns in children from in vitro fertilization or intracytoplasmic sperm injection

Objective - To study the possible transmission, to the next generation, of epigenetic defects associated with in vitro maturation (IVM) of human oocytes. - Design - Case-control study using epigenetic data. - Setting - Two collaborating university departments. - Patient(s) - Eleven IVM newborns and...

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Main Authors: Pliushch, Galyna (Author) , Schneider, Eberhard (Author) , Schneider, Tamara (Author) , El Hajj, Nady (Author) , Rösner, Sabine (Author) , Strowitzki, Thomas (Author) , Haaf, Thomas (Author)
Format: Article (Journal)
Language:English
Published: January 6, 2015
In: Fertility and sterility
Year: 2015, Volume: 103, Issue: 3, Pages: 720-727.e1
ISSN:1556-5653
DOI:10.1016/j.fertnstert.2014.12.096
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.fertnstert.2014.12.096
Verlag, lizenzpflichtig, Volltext: http://www.sciencedirect.com/science/article/pii/S0015028214025217
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Author Notes:Galyna Pliushch, M.Sc., Eberhard Schneider, Ph.D., Tamara Schneider, Ph.D., Nady El Hajj, Ph.D.,Sabine Rösner, M.D., Thomas Strowitzki, M.D., and Thomas Haaf, M.D.
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Summary:Objective - To study the possible transmission, to the next generation, of epigenetic defects associated with in vitro maturation (IVM) of human oocytes. - Design - Case-control study using epigenetic data. - Setting - Two collaborating university departments. - Patient(s) - Eleven IVM newborns and 19 controls, conceived by conventional assisted reproduction. - Intervention(s) - Chorionic villus and cord-blood sampling. - Main Outcome Measure(s) - Using bisulfite pyrosequencing, we have measured average methylation levels of 6 imprinted (LIT1, MEG, MEST, NESPas, PEG3, and SNRPN), 5 tumor-suppressor (APC, ATM, BRCA1, RAD51C, and TP53), 2 pluripotency (NANOG and OCT4), and 2 metabolic (LEP and NR3C1) genes, as well as 2 repetitive elements (ALU and LINE1) in 2 tissues of IVM and control neonates. Using deep bisulfite sequencing, we have determined methylation patterns of many individual DNA molecules to detect rare RAD51C epimutations (allele methylation errors). - Result(s) - No statistically significant impact was found of IVM on chorionic villus and cord-blood DNA methylation at the studied developmentally important genes and interspersed repeats. The RAD51C epimutation rate was low (0.5% ± 0.1%) in all analyzed samples. - Conclusion(s) - IVM-induced epigenetic changes in offspring, if any, are relatively small in magnitude and/or infrequent.
Item Description:Gesehen am 08.07.2020
Physical Description:Online Resource
ISSN:1556-5653
DOI:10.1016/j.fertnstert.2014.12.096

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