Cover Image

Methylation of ribosomal RNA by NSUN5 is a conserved mechanism modulating organismal lifespan

Several pathways modulating longevity and stress resistance converge on translation by targeting ribosomal proteins or initiation factors, but whether this involves modifications of ribosomal RNA is unclear. Here, we show that reduced levels of the conserved RNA methyltransferase NSUN5 increase the...

Full description

Saved in:
Bibliographic Details
Main Authors: Schosserer, Markus (Author) , Minois, Nadege (Author) , Angerer, Tina B. (Author) , Amring, Manuela (Author) , Dellago, Hanna (Author) , Harreither, Eva (Author) , Calle-Perez, Alfonso (Author) , Pircher, Andreas (Author) , Gerstl, Matthias Peter (Author) , Pfeifenberger, Sigrid (Author) , Brandl, Clemens (Author) , Sonntagbauer, Markus (Author) , Kriegner, Albert (Author) , Linder, Angela (Author) , Weinhäusel, Andreas (Author) , Mohr, Thomas (Author) , Steiger, Matthias (Author) , Mattanovich, Diethard (Author) , Rinnerthaler, Mark (Author) , Karl, Thomas (Author) , Sharma, Sunny (Author) , Entian, Karl-Dieter (Author) , Koš, Martin (Author) , Breitenbach, Michael (Author) , Wilson, Iain B. H. (Author) , Polacek, Norbert (Author) , Grillari-Voglauer, Regina (Author) , Breitenbach-Koller, Hannelore (Author) , Grillari, Johannes (Author)
Format: Article (Journal)
Language:English
Published: 30 January 2015
In: Nature Communications
Year: 2015, Volume: 6
ISSN:2041-1723
DOI:10.1038/ncomms7158
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/ncomms7158
Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/ncomms7158
Get full text
Author Notes:Markus Schosserer, Nadege Minois, Tina B. Angerer, Manuela Amring, Hanna Dellago, Eva Harreither, Alfonso Calle-Perez, Andreas Pircher, Matthias Peter Gerstl, Sigrid Pfeifenberger, Clemens Brandl, Markus Sonntagbauer, Albert Kriegner, Angela Linder, Andreas Weinhäusel, Thomas Mohr, Matthias Steiger, Diethard Mattanovich, Mark Rinnerthaler, Thomas Karl, Sunny Sharma, Karl-Dieter Entian, Martin Kos, Michael Breitenbach, Iain B. H. Wilson, Norbert Polacek, Regina Grillari-Voglauer, Lore Breitenbach-Koller & Johannes Grillari
Description
Summary:Several pathways modulating longevity and stress resistance converge on translation by targeting ribosomal proteins or initiation factors, but whether this involves modifications of ribosomal RNA is unclear. Here, we show that reduced levels of the conserved RNA methyltransferase NSUN5 increase the lifespan and stress resistance in yeast, worms and flies. Rcm1, the yeast homologue of NSUN5, methylates C2278 within a conserved region of 25S rRNA. Loss of Rcm1 alters the structural conformation of the ribosome in close proximity to C2278, as well as translational fidelity, and favours recruitment of a distinct subset of oxidative stress-responsive mRNAs into polysomes. Thus, rather than merely being a static molecular machine executing translation, the ribosome exhibits functional diversity by modification of just a single rRNA nucleotide, resulting in an alteration of organismal physiological behaviour, and linking rRNA-mediated translational regulation to modulation of lifespan, and differential stress response.
Item Description:Gesehen am 13.07.2020
Physical Description:Online Resource
ISSN:2041-1723
DOI:10.1038/ncomms7158

Similar Items