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Impact of sodium butyrate on the network of adhesion/growth-regulatory galectins in human colon cancer in vitro

Background/Aim: The physiological compound sodium butyrate can induce differentiation in colon cancer cells in vitro. Due to the role of galectins in growth control we explored its effect on this network beyond galectins-1 and -3, with deliberate consideration of the status of microsatellite stabili...

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Bibliographic Details
Main Authors: Katzenmaier, Eva-Maria Katharina (Author) , André, Sabine (Author) , Kopitz, Jürgen (Author) , Gabius, Hans-Joachim (Author)
Format: Article (Journal)
Language:English
Published: October 2014
In: Anticancer research
Year: 2014, Volume: 34, Issue: 10, Pages: 5429-5438
ISSN:1791-7530
Online Access:Verlag, lizenzpflichtig, Volltext: http://ar.iiarjournals.org/content/34/10/5429
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Author Notes:Eva-Maria Katzenmaier, Sabine André, Jürgen Kopitz and Hans-Joachim Gabius
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Summary:Background/Aim: The physiological compound sodium butyrate can induce differentiation in colon cancer cells in vitro. Due to the role of galectins in growth control we explored its effect on this network beyond galectins-1 and -3, with deliberate consideration of the status of microsatellite stability, for nine cell lines. Materials and Methods: Microscopical monitoring and measurement of alkaline phosphatase activity ascertained butyrate's impact on cells. Monitoring by reverse transcriptase-polymerase chain reaction (RT-PCR) and western blotting with galectin-type-specific probes characterized galectin expression. Results: Controlled by expectable strong up-regulation of galectin-1 and comparatively small effects on galectin-3 regulation for galectins-4, -7, -8 and -9 were reported with no obvious association to microsatellite stability status. Neoexpression of the GAL-12 gene was observed in eight out of nine tested lines. Conclusion: Butyrate affects the galectin network beyond galectins-1 and -3, warranting further cell biological and histochemical studies.
Item Description:Gesehen am 20.07.2020
Physical Description:Online Resource
ISSN:1791-7530

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