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Nanoparticle tension probes patterned at the nanoscale: impact of integrin clustering on force transmission

Herein we aimed to understand how nanoscale clustering of RGD ligands alters the mechano-regulation of their integrin receptors. We combined molecular tension fluorescence microscopy with block copolymer micelle nanolithography to fabricate substrates with arrays of precisely spaced probes that can...

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Bibliographic Details
Main Authors: Liu, Yang (Author) , Medda, Rebecca (Author) , Liu, Zheng (Author) , Galior, Kornelia (Author) , Yehl, Kevin (Author) , Spatz, Joachim P. (Author) , Cavalcanti-Adam, Elisabetta A. (Author) , Salaita, Khalid (Author)
Format: Article (Journal)
Language:English
Published: September 19, 2014
In: Nano letters
Year: 2014, Volume: 14, Issue: 10, Pages: 5539-5546
ISSN:1530-6992
DOI:10.1021/nl501912g
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1021/nl501912g
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Author Notes:Yang Liu, Rebecca Medda, Zheng Liu, Kornelia Galior, Kevin Yehl, Joachim P. Spatz, Elisabetta Ada Cavalcanti-Adam, and Khalid Salaita
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Summary:Herein we aimed to understand how nanoscale clustering of RGD ligands alters the mechano-regulation of their integrin receptors. We combined molecular tension fluorescence microscopy with block copolymer micelle nanolithography to fabricate substrates with arrays of precisely spaced probes that can generate a 10-fold fluorescence response to pN-forces. We found that the mechanism of sensing ligand spacing is force-mediated. This strategy is broadly applicable to investigating receptor clustering and its role in mechanotransduction pathways.
Item Description:Gesehen am 20.07.2020
Physical Description:Online Resource
ISSN:1530-6992
DOI:10.1021/nl501912g

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