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Nanoscale control of surface immobilized BMP-2: toward a quantitative assessment of BMP-mediated signaling vents

In this work we determine the impact of surface density of immobilized BMP-2 on intracellular signal transduction. We use block copolymer micellar nanolithography to fabricate substrates with precisely spaced and tunable gold nanoparticle arrays carrying single BMP-2 molecules. We found that the imm...

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Bibliographic Details
Main Authors: Baha-Schwab, Elisabeth H. (Author) , Pohl, Theresa Lucie Maria (Author) , Haraszti, Tamás (Author) , Schwaerzer, Gerburg K. (Author) , Hiepen, Christian (Author) , Spatz, Joachim P. (Author) , Knaus, Petra (Author) , Cavalcanti-Adam, Elisabetta A. (Author)
Format: Article (Journal)
Language:English
Published: February 10, 2015
In: Nano letters
Year: 2015, Volume: 15, Issue: 3, Pages: 1526-1534
ISSN:1530-6992
DOI:10.1021/acs.nanolett.5b00315
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1021/acs.nanolett.5b00315
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Author Notes:Elisabeth H. Schwab, Theresa L.M. Pohl, Tamás Haraszti, Gerburg K. Schwaerzer, Christian Hiepen, Joachim P. Spatz, Petra Knaus, and Elisabetta A. Cavalcanti-Adam
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Summary:In this work we determine the impact of surface density of immobilized BMP-2 on intracellular signal transduction. We use block copolymer micellar nanolithography to fabricate substrates with precisely spaced and tunable gold nanoparticle arrays carrying single BMP-2 molecules. We found that the immobilized growth factor triggers prolonged and elevated Smad signaling pathway activation compared to the same amount of soluble protein. This approach is suitable for achieving controlled and sustained local delivery of BMP-2 and other growth factors.
Item Description:Gesehen am 21.07.2020
Physical Description:Online Resource
ISSN:1530-6992
DOI:10.1021/acs.nanolett.5b00315

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