Genetic variants in apoptosis-related genes associated with colorectal hyperplasia
Deregulation of apoptosis is a frequent alteration in early benign lesions of the colon mucosa and is thought to be a major contributor to tumor progression and cancer. Single nucleotide polymorphisms (SNPs) within apoptosis-related genes could affect apoptotic responses and their identification mig...
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| Main Authors: | , , , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
27 May 2014
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| In: |
Genes, chromosomes & cancer
Year: 2014, Volume: 53, Issue: 9, Pages: 769-778 |
| ISSN: | 1098-2264 |
| DOI: | 10.1002/gcc.22185 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1002/gcc.22185 Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/gcc.22185 
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| Author Notes: | Stefano Gerola, Stefanie Nittka, Georg Kähler, Sha Tao, Hermann Brenner, Giorgio Binelli, Roland Eils, Benedikt Brors, and Michael Neumaier |
| Summary: | Deregulation of apoptosis is a frequent alteration in early benign lesions of the colon mucosa and is thought to be a major contributor to tumor progression and cancer. Single nucleotide polymorphisms (SNPs) within apoptosis-related genes could affect apoptotic responses and their identification might provide a basis to assess individual risk for development of early lesions. To investigate a possible association between genetic polymorphisms and the occurrence of hyperplastic polyps (HP), we developed a custom DNA chip assay for 1,536 SNPs in the coding and flanking regions of 826 genes with known functional roles in apoptosis or apoptosis-associated (e.g., stress-related) pathways. During a first round of screening, genotypes were determined for 272 endoscopy patients harboring hyperplastic colorectal polyps and for 512 sex and aged-matched controls. A set of 14 candidate SNPs associated with HP (P < 0.01) was then evaluated in an independent cohort of patients (n = 38) and controls (n = 38). Following meta-analysis of Stages I and II, a false discovery rate approach was applied. Among the 14 candidate SNPs, eight showed significant association (combined P < 0.01) with the occurrence of HP. The SNPs rs4709583 (PARK2) and rs10476823 (HDAC3) were analyzed for potential functional effects on RNA splicing and RNA half-life. Despite its location near a splice site, alternative splicing was not detected for rs4709583 (PARK3). By contrast, cDNA analysis revealed use of a cryptic polyadenylation signal in the 3′UTR of HDAC3 mRNA and a longer mRNA half-life in a cell line heterozygous for rs10476823. © 2014 Wiley Periodicals, Inc. |
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| Item Description: | Gesehen am 27.07.2020 |
| Physical Description: | Online Resource |
| ISSN: | 1098-2264 |
| DOI: | 10.1002/gcc.22185 |