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Non-circadian expression masking clock-driven weak transcription rhythms in U2OS cells

U2OS cells harbor a circadian clock but express only a few rhythmic genes in constant conditions. We identified 3040 binding sites of the circadian regulators BMAL1, CLOCK and CRY1 in the U2OS genome. Most binding sites even in promoters do not correlate with detectable rhythmic transcript levels. L...

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Bibliographic Details
Main Authors: Hoffmann, Julia (Author) , Shostak, Anton (Author) , Fischer, Tamás (Author) , Brunner, Michael (Author)
Format: Article (Journal)
Language:English
Published: July 9, 2014
In: PLOS ONE
Year: 2014, Volume: 9, Issue: 7
ISSN:1932-6203
DOI:10.1371/journal.pone.0102238
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1371/journal.pone.0102238
Verlag, lizenzpflichtig, Volltext: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0102238
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Author Notes:Julia Hoffmann, Laura Symul, Anton Shostak, Tamás Fischer, Felix Naef, Michael Brunner
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Summary:U2OS cells harbor a circadian clock but express only a few rhythmic genes in constant conditions. We identified 3040 binding sites of the circadian regulators BMAL1, CLOCK and CRY1 in the U2OS genome. Most binding sites even in promoters do not correlate with detectable rhythmic transcript levels. Luciferase fusions reveal that the circadian clock supports robust but low amplitude transcription rhythms of representative promoters. However, rhythmic transcription of these potentially clock-controlled genes is masked by non-circadian transcription that overwrites the weaker contribution of the clock in constant conditions. Our data suggest that U2OS cells harbor an intrinsically rather weak circadian oscillator. The oscillator has the potential to regulate a large number of genes. The contribution of circadian versus non-circadian transcription is dependent on the metabolic state of the cell and may determine the apparent complexity of the circadian transcriptome.
Item Description:Gesehen am 06.08.2020
Physical Description:Online Resource
ISSN:1932-6203
DOI:10.1371/journal.pone.0102238

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