Osteogenic and chondrogenic potential of the supramolecular aggregate T-LysYal®
Hard tissue regeneration represents a challenge for the Regenerative Medicine and Mesenchymal stem cells (MSCs) could be a successful therapeutic strategy. T-LysYal® (T-Lys), a new derivative of Hyaluronic Acid (HA) possessing a superior stability, has already been proved efficient in repairing corn...
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| Main Authors: | , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
05 May 2020
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| In: |
Frontiers in endocrinology
Year: 2020, Volume: 11 |
| ISSN: | 1664-2392 |
| DOI: | 10.3389/fendo.2020.00285 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.3389/fendo.2020.00285 Verlag, lizenzpflichtig, Volltext: https://www.frontiersin.org/articles/10.3389/fendo.2020.00285/full 
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| Author Notes: | Adriana Di Benedetto, Francesca Posa, Mario Marazzi, Zamira Kalemaj, Roberta Grassi, Lorenzo Lo Muzio, Mariasevera Di Comite, Elisabetta Ada Cavalcanti-Adam, Felice Roberto Grassi and Giorgio Mori |
| Summary: | Hard tissue regeneration represents a challenge for the Regenerative Medicine and Mesenchymal stem cells (MSCs) could be a successful therapeutic strategy. T-LysYal® (T-Lys), a new derivative of Hyaluronic Acid (HA) possessing a superior stability, has already been proved efficient in repairing corneal epithelial cells damaged by dry conditions in vitro. We investigated the regenerative potential of T-Lys in the hard tissues bone and cartilage. We have previously demonstrated that cells isolated from the tooth germ, Dental Bud Stem Cells (DBSCs), differentiate into osteoblast-like cells, representing a promising source of MSCs for bone regeneration. Herewith, we show that T-Lys treatment stimulates the expression of typical osteoblastic markers, such as Alkaline Phosphatase (ALP) and Osteopontin (OPN), determining a higher production of mineralized matrix nodules. In addition, we found that T-Lys treatment positively affects αVβ3 integrin expression, key integrin in the osteoblastic commitment, leading to the formation of focal adhesions (FAs). The efficacy of T-Lys was also tested on chondrogenic differentiation starting from human articular chondrocytes (HACs) resulting in an increase of differentiation markers and cell number. |
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| Item Description: | Gesehen am 13.08.2020 |
| Physical Description: | Online Resource |
| ISSN: | 1664-2392 |
| DOI: | 10.3389/fendo.2020.00285 |