Semiautomated correlative 3D electron microscopy of in vivo- imaged axons and dendrites

The authors describe an approach that can be used after in vivo imaging of dendrites and axons in adult mouse brains to prepare and image fluorescent structures of interest using focussed ion beam scanning electron microscopy.

Saved in:
Bibliographic Details
Main Authors: Maco, Bohumil (Author) , Cantoni, Marco (Author) , Holtmaat, Anthony (Author) , Kreshuk, Anna (Author) , Hamprecht, Fred (Author) , Knott, Graham W. (Author)
Format: Article (Journal)
Language:English
Published: 15 May 2014
In: Nature protocols
Year: 2014, Volume: 9, Issue: 6, Pages: 1354-1366
ISSN:1750-2799
DOI:10.1038/nprot.2014.101
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/nprot.2014.101
Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/nprot.2014.101
Get full text
Author Notes:Bohumil Maco, Marco Cantoni, Anthony Holtmaat, Anna Kreshuk, Fred A. Hamprecht and Graham W. Knott
Description
Summary:The authors describe an approach that can be used after in vivo imaging of dendrites and axons in adult mouse brains to prepare and image fluorescent structures of interest using focussed ion beam scanning electron microscopy.
Item Description:Gesehen am 18.08.2020
Physical Description:Online Resource
ISSN:1750-2799
DOI:10.1038/nprot.2014.101