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Proteomics of vitreous humor of patients with exudative age-related macular degeneration

Background There is absence of specific biomarkers and an incomplete understanding of the pathophysiology of exudative age-related macular degeneration (AMD). Methods and Findings Eighty-eight vitreous samples (73 from patients with treatment naïve AMD and 15 control samples from patients with idio...

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Main Authors: Koss, Michael Janusz (Author) , Hoffmann, Janosch (Author) , Nguyen, Nauke (Author) , Pfister, Marcel (Author) , Mischak, Harald (Author) , Mullen, William (Author) , Husi, Holger (Author) , Rejdak, Robert (Author) , Koch, Frank (Author) , Jankowski, Joachim (Author) , Krueger, Katharina (Author) , Bertelmann, Thomas (Author) , Klein, Julie (Author) , Schanstra, Joost P. (Author) , Siwy, Justyna (Author)
Format: Article (Journal)
Language:English
Published: May 14, 2014
In: PLOS ONE
Year: 2014, Volume: 9, Issue: 5
ISSN:1932-6203
DOI:10.1371/journal.pone.0096895
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1371/journal.pone.0096895
Verlag, lizenzpflichtig, Volltext: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0096895
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Author Notes:Michael Janusz Koss, Janosch Hoffmann, Nauke Nguyen, Marcel Pfister, Harald Mischak, William Mullen, Holger Husi, Robert Rejdak, Frank Koch, Joachim Jankowski, Katharina Krueger, Thomas Bertelmann, Julie Klein, Joost P. Schanstra, Justyna Siwy
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Summary:Background There is absence of specific biomarkers and an incomplete understanding of the pathophysiology of exudative age-related macular degeneration (AMD). Methods and Findings Eighty-eight vitreous samples (73 from patients with treatment naïve AMD and 15 control samples from patients with idiopathic floaters) were analyzed with capillary electrophoresis coupled to mass spectrometry in this retrospective case series to define potential candidate protein markers of AMD. Nineteen proteins were found to be upregulated in vitreous of AMD patients. Most of the proteins were plasma derived and involved in biological (ion) transport, acute phase inflammatory reaction, and blood coagulation. A number of proteins have not been previously associated to AMD including alpha-1-antitrypsin, fibrinogen alpha chain and prostaglandin H2-D isomerase. Alpha-1-antitrypsin was validated in vitreous of an independent set of AMD patients using Western blot analysis. Further systems biology analysis of the data indicated that the observed proteomic changes may reflect upregulation of immune response and complement activity. Conclusions Proteome analysis of vitreous samples from patients with AMD, which underwent an intravitreal combination therapy including a core vitrectomy, steroids and bevacizumab, revealed apparent AMD-specific proteomic changes. The identified AMD-associated proteins provide some insight into the pathophysiological changes associated with AMD.
Item Description:Gesehen am 19.08.2020
Physical Description:Online Resource
ISSN:1932-6203
DOI:10.1371/journal.pone.0096895

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