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Bacterial lipopolysaccharides form physically cross-linked, two-dimensional gels in the presence of divalent cations

We established a bacterial membrane model with monolayers of bacterial lipopolysaccharides (LPS Re and LPS Ra) and quantified their viscoelastic properties by using an interfacial stress rheometer coupled to a Langmuir film balance. LPS Re monolayers exhibited purely viscous behaviour in the absence...

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Bibliographic Details
Main Authors: Herrmann, Moritz (Author) , Schneck, Emanuel (Author) , Gutsmann, Thomas (Author) , Brandenburg, Klaus (Author) , Tanaka, Motomu (Author)
Format: Article (Journal)
Language:English
Published: 02 Jul 2015
In: Soft matter
Year: 2015, Volume: 11, Issue: 30, Pages: 6037-6044
ISSN:1744-6848
DOI:10.1039/C5SM01002K
Online Access:Verlag, Volltext: https://doi.org/10.1039/C5SM01002K
Verlag: https://pubs.rsc.org/en/content/articlelanding/2015/sm/c5sm01002k
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Author Notes:Moritz Herrmann, Emanuel Schneck, Thomas Gutsmann, Klaus Brandenburg, Motomu Tanaka
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Summary:We established a bacterial membrane model with monolayers of bacterial lipopolysaccharides (LPS Re and LPS Ra) and quantified their viscoelastic properties by using an interfacial stress rheometer coupled to a Langmuir film balance. LPS Re monolayers exhibited purely viscous behaviour in the absence of calcium ions, while the same monolayers underwent a viscous-to-elastic transition upon compression in the presence of Ca2+. Our results demonstrated for the first time that LPSs in bacterial outer membranes can form two-dimensional elastic networks in the presence of Ca2+. Different from LPS Re monolayers, the LPS Ra monolayers showed a very similar rheological transition both in the presence and absence of Ca2+, suggesting that longer saccharide chains can form 2D physical gels even in the absence of Ca2+. By exposure of the monolayers to the antimicrobial peptide protamine, we could directly monitor the differences in resistance of bacterial membranes according to the presence of calcium.
Item Description:Gesehen am 27.08.2020
Physical Description:Online Resource
ISSN:1744-6848
DOI:10.1039/C5SM01002K

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