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Support and challenges to the melanosomal casing model based on nanoscale distribution of metals within iris melanosomes detected by X-ray fluorescence analysis

Melanin within melanosomes exists as eumelanin or pheomelanin. Distributions of these melanins have been studied extensively within tissues, but less often within individual melanosomes. Here, we apply X-ray fluorescence analysis with synchrotron radiation to survey the nanoscale distribution of met...

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Main Authors: Gorniak, Thomas (Author) , Haraszti, Tamás (Author) , Suhonen, Heikki (Author) , Yang, Yang (Author) , Hedberg‐Buenz, Adam (Author) , Koehn, Demelza (Author) , Heine, Ruth (Author) , Grunze, Michael (Author) , Rosenhahn, Axel (Author) , Anderson, Michael G. (Author)
Format: Article (Journal)
Language:English
Published: 5 June 2014
In: Pigment cell & melanoma research
Year: 2014, Volume: 27, Issue: 5, Pages: 831-834
ISSN:1755-148X
DOI:10.1111/pcmr.12278
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1111/pcmr.12278
Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1111/pcmr.12278
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Author Notes:Thomas Gorniak, Tamás Haraszti, Heikki Suhonen, Yang Yang, Adam Hedberg‐Buenz, Demelza Koehn, Ruth Heine, Michael Grunze, Axel Rosenhahn and Michael G. Anderson
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Summary:Melanin within melanosomes exists as eumelanin or pheomelanin. Distributions of these melanins have been studied extensively within tissues, but less often within individual melanosomes. Here, we apply X-ray fluorescence analysis with synchrotron radiation to survey the nanoscale distribution of metals within purified melanosomes of mice. The study allows a discovery-based characterization of melanosomal metals, and, because Cu is specifically associated with eumelanin, a hypothesis-based test of the ‘casing model’ predicting that melanosomes contain a pheomelanin core surrounded by a eumelanin shell. Analysis of Cu, Ca, and Zn shows variable concentrations and distributions, with Ca/Zn highly correlated, and at least three discrete patterns for the distribution of Cu vs. Ca/Zn in different melanosomes - including one with a Cu-rich shell surrounding a Ca/Zn-rich core. Thus, the results support predictions of the casing model, but also suggest that in at least some tissues and genetic contexts, other arrangements of melanin may co-exist.
Item Description:Gesehen am 04.09.2020
Physical Description:Online Resource
ISSN:1755-148X
DOI:10.1111/pcmr.12278

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