Cover Image

Magnetically guided recellularization of decellularized stented porcine pericardium-derived aortic valve for TAVI

Application of somatic stem cells for growth, proliferation, and differentiation in a three-dimensional pattern is an important aspect in tissue engineering. Here, we report on our bioreactor, which we applied for magnetically guided recellularization of nitinol-stented valve. Human-derived unrestri...

Full description

Saved in:
Bibliographic Details
Main Authors: Ghodsizad, Ali (Author) , Bordel, Viktor (Author) , Wiedensohler, Hermann (Author) , Elbanayosy, Ali (Author) , Koerner, Michael Martin (Author) , Gonzalez Berjon, Jose M. (Author) , Barrios, Roberto (Author) , Farag, Mina (Author) , Zeriouh, Mohamad (Author) , Loebe, Matthias (Author) , Noon, George Peter (Author) , Koegler, Gesine (Author) , Karck, Matthias (Author) , Ruhparwar, Arjang (Author)
Format: Article (Journal)
Language:English
Published: [2014]
In: ASAIO journal
Year: 2014, Volume: 60, Issue: 5, Pages: 582-586
ISSN:1538-943X
DOI:10.1097/MAT.0000000000000110
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1097/MAT.0000000000000110
Verlag, lizenzpflichtig, Volltext: https://journals.lww.com/asaiojournal/Fulltext/2014/09000/Magnetically_Guided_Recellularization_of.17.aspx
Get full text
Author Notes:Ali Ghodsizad, Viktor Bordel, Herrman Wiedensohler, Ali Elbanayosy, Michael Martin Koerner, Jose M. Gonzalez Berjon, Roberto Barrios, Mina Farag, Mohamad Zeriouh, Matthias Loebe, George Peter Noon, Gesine Koegler, Matthias Karck, and Arjang Ruhparwar
Description
Summary:Application of somatic stem cells for growth, proliferation, and differentiation in a three-dimensional pattern is an important aspect in tissue engineering. Here, we report on our bioreactor, which we applied for magnetically guided recellularization of nitinol-stented valve. Human-derived unrestricted somatic stem cells were cultured in medium in our pulsatile dynamic bioreactor for 4-6 days. Stented valves were prepared by decellularization of porcine pericardium and construction of stented tissue-engineered valves (n = 8). A magnetic field was created around the bioreactor to prevent the loss of cells. In the control group, no magnetic device was used (n = 4). Morphological characterization was assessed by immunohistochemical staining of paraffin sections and electron microscopy. The bioreactor enabled the preservation of physiologic culture conditions with aerobic cell metabolism and physiological pH values. Histological analysis showed homogeneous seeding of the pericardium with progenitor cells in the recellularized samples, whereas no cell seeding could be observed in the nonmagnetic group. Our magnetically guided multifunctional bioreactor allows for an efficient three-dimensional culturing of somatic stem cells on decellularized organ-specific matrix.
Item Description:Gesehen am 10.09.2020
Physical Description:Online Resource
ISSN:1538-943X
DOI:10.1097/MAT.0000000000000110

Similar Items