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Immune escape and survival mechanisms in circulating tumor cells of colorectal cancer

The prognosis of colorectal cancer is closely linked to the occurrence of distant metastases. Systemic dissemination is most likely caused by circulating tumor cells (CTC). Despite the fundamental role of CTC within the metastatic cascade, technical obstacles have so far prevented detailed genomic a...

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Main Authors: Steinert, Gunnar (Author) , Schölch, Sebastian (Author) , Niemietz, Thomas (Author) , Iwata, Naoki (Author) , García, Sebastián A. (Author) , Behrens, Bianca (Author) , Voigt, Anita Yvonne (Author) , Kloor, Matthias (Author) , Benner, Axel (Author) , Bork, Ulrich (Author) , Rahbari, Nuh N. (Author) , Büchler, Markus W. (Author) , Stoecklein, Nikolas H. (Author) , Weitz, Jürgen (Author) , Koch, Moritz (Author)
Format: Article (Journal)
Language:English
Published: March 5, 2014
In: Cancer research
Year: 2014, Volume: 74, Issue: 6, Pages: 1694-1704
ISSN:1538-7445
DOI:10.1158/0008-5472.CAN-13-1885
Online Access:Resolving-System, lizenzpflichtig, Volltext: https://doi.org/10.1158/0008-5472.CAN-13-1885
Verlag, lizenzpflichtig, Volltext: https://cancerres.aacrjournals.org/content/74/6/1694
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Author Notes:Gunnar Steinert, Sebastian Schölch, Thomas Niemietz, Naoki Iwata, Sebastián A. García, Bianca Behrens, Anita Voigt, Matthias Kloor, Axel Benner, Ulrich Bork, Nuh N. Rahbari, Markus W. Büchler, Nikolas H. Stoecklein, Jürgen Weitz, and Moritz Koch
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Summary:The prognosis of colorectal cancer is closely linked to the occurrence of distant metastases. Systemic dissemination is most likely caused by circulating tumor cells (CTC). Despite the fundamental role of CTC within the metastatic cascade, technical obstacles have so far prevented detailed genomic and, in particular, phenotypic analyses of CTC, which may provide molecular targets to delay or prevent distant metastases. We show here a detailed genomic analysis of single colorectal cancer-derived CTC by array comparative genomic hybridization (aCGH), mutational profiling, and microsatellite instability (MSI) analysis. Furthermore, we report the first gene expression analysis of manually selected colorectal cancer-derived CTC by quantitative real-time PCR (qRT-PCR) to investigate transcriptional changes, enabling CTC to survive in circulation and form distant metastases.
Item Description:Gesehen am 22.09.2020
Physical Description:Online Resource
ISSN:1538-7445
DOI:10.1158/0008-5472.CAN-13-1885

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