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Alternative anesthesia of neonatal mice for global rAAV delivery in the brain with non-detectable behavioral interference in adults

Viral transduced gene expression is the current standard for cell-type specific labelling and cell tacking in experimental neuroscience. To achieve widespread gene expression, a viral delivery method to neonatal rodents was introduced more than two decades ago. Most of those neonatal virus-injection...

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Bibliographic Details
Main Authors: Tang, Wannan (Author) , Zillmann, Uwe (Author) , Sprengel, Rolf (Author)
Format: Article (Journal)
Language:English
Published: 14 July 2020
In: Frontiers in behavioral neuroscience
Year: 2020, Volume: 14
ISSN:1662-5153
DOI:10.3389/fnbeh.2020.00115
Online Access:Verlag, Volltext: https://doi.org/10.3389/fnbeh.2020.00115
Verlag, Volltext: https://www.frontiersin.org/articles/10.3389/fnbeh.2020.00115/full
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Author Notes:Wannan Tang, Uwe Zillmann and Rolf Sprengel
Description
Summary:Viral transduced gene expression is the current standard for cell-type specific labelling and cell tacking in experimental neuroscience. To achieve widespread gene expression, a viral delivery method to neonatal rodents was introduced more than two decades ago. Most of those neonatal virus-injection based gene transduction methods in mice have used deep hypothermia for anesthesia, which was reported to be associated with behavioral impairments in respective animals. To explore other options for neonatal viral applications, we applied a combination of Medetomidine, Midazolam and Fentanyl (MMF), each of which can be antagonized by a specific antagonist. Later in their adulthood, drug treated, virus injected mice and naïve mice showed a similar performance in all behavioral tasks tested, including motor coordination tasks, anxiety-related tasks and spatial memory tasks, demonstrating MMF anesthesia could be safely applied to mice for neonatal viral transduction at postnatal day 2.
Item Description:Gesehen am 28.09.2020
Physical Description:Online Resource
ISSN:1662-5153
DOI:10.3389/fnbeh.2020.00115

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