RNA-peptide conjugate synthesis by inverse-electron demand Diels-Alder reaction
Here we report an efficient method for the synthesis of RNA-peptide conjugates by inverse-electron demand Diels-Alder reaction. Various dienophiles were enzymatically incorporated into RNA and reacted with a chemically synthesized diene-modified peptide. The Diels-Alder reaction proceeds with near-q...
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| Main Authors: | , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
20 May 2014
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| In: |
Organic & biomolecular chemistry
Year: 2014, Volume: 12, Issue: 26, Pages: 4701-4707 |
| ISSN: | 1477-0539 |
| DOI: | 10.1039/C4OB00076E |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1039/C4OB00076E Verlag, lizenzpflichtig, Volltext: https://pubs.rsc.org/en/content/articlelanding/2014/ob/c4ob00076e 
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| Author Notes: | Sandeep Ameta, Juliane Becker and Andres Jäschke |
| Summary: | Here we report an efficient method for the synthesis of RNA-peptide conjugates by inverse-electron demand Diels-Alder reaction. Various dienophiles were enzymatically incorporated into RNA and reacted with a chemically synthesized diene-modified peptide. The Diels-Alder reaction proceeds with near-quantitative yields in aqueous solution with stoichiometric amounts of reactants, even at low micromolar concentrations. |
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| Item Description: | Gesehen am 30.09.2020 |
| Physical Description: | Online Resource |
| ISSN: | 1477-0539 |
| DOI: | 10.1039/C4OB00076E |