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RNA-peptide conjugate synthesis by inverse-electron demand Diels-Alder reaction

Here we report an efficient method for the synthesis of RNA-peptide conjugates by inverse-electron demand Diels-Alder reaction. Various dienophiles were enzymatically incorporated into RNA and reacted with a chemically synthesized diene-modified peptide. The Diels-Alder reaction proceeds with near-q...

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Hauptverfasser: Ameta, Sandeep (Verfasst von) , Becker, Juliane (Verfasst von) , Jäschke, Andres (Verfasst von)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 20 May 2014
In: Organic & biomolecular chemistry
Year: 2014, Jahrgang: 12, Heft: 26, Pages: 4701-4707
ISSN:1477-0539
DOI:10.1039/C4OB00076E
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1039/C4OB00076E
Verlag, lizenzpflichtig, Volltext: https://pubs.rsc.org/en/content/articlelanding/2014/ob/c4ob00076e
Volltext
Verfasserangaben:Sandeep Ameta, Juliane Becker and Andres Jäschke
Beschreibung
Zusammenfassung:Here we report an efficient method for the synthesis of RNA-peptide conjugates by inverse-electron demand Diels-Alder reaction. Various dienophiles were enzymatically incorporated into RNA and reacted with a chemically synthesized diene-modified peptide. The Diels-Alder reaction proceeds with near-quantitative yields in aqueous solution with stoichiometric amounts of reactants, even at low micromolar concentrations.
Beschreibung:Gesehen am 30.09.2020
Beschreibung:Online Resource
ISSN:1477-0539
DOI:10.1039/C4OB00076E

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