Cover Image

The Archaeal Proteome Project advances knowledge about archaeal cell biology through comprehensive proteomics

While many aspects of archaeal cell biology remain relatively unexplored, systems biology approaches like mass spectrometry (MS) based proteomics offer an opportunity for rapid advances. Unfortunately, the enormous amount of MS data generated often remains incompletely analyzed due to a lack of soph...

Full description

Saved in:
Bibliographic Details
Main Authors: Schulze, Stefan (Author) , Adams, Zachary (Author) , Cerletti, Micaela (Author) , De Castro, Rosana (Author) , Ferreira-Cerca, Sebastien (Author) , Fufezan, Christian (Author) , Gimenez, Maria Ines (Author) , Hippler, Michael (Author) , Jevtic, Zivojin (Author) , Knüppel, Robert (Author) , Legerme, Georgio (Author) , Lenz, Christof (Author) , Marchfelder, Anita (Author) , Maupin-Furlow, Julie (Author) , Paggi, Roberto A. (Author) , Pfeiffer, Friedhelm (Author) , Poetsch, Ansgar (Author) , Urlaub, Henning (Author) , Pohlschroder, Mechthild (Author)
Format: Article (Journal)
Language:English
Published: 19 June 2020
In: Nature Communications
Year: 2020, Volume: 11
ISSN:2041-1723
DOI:10.1038/s41467-020-16784-7
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/s41467-020-16784-7
Get full text
Author Notes:Stefan Schulze, Zachary Adams, Micaela Cerletti, Rosana De Castro, Sébastien Ferreira-Cerca, Christian Fufezan, María Inés Giménez, Michael Hippler, Zivojin Jevtic, Robert Knüppel, Georgio Legerme, Christof Lenz, Anita Marchfelder, Julie Maupin-Furlow, Roberto A. Paggi, Friedhelm Pfeiffer, Ansgar Poetsch, Henning Urlaub & Mechthild Pohlschroder
Description
Summary:While many aspects of archaeal cell biology remain relatively unexplored, systems biology approaches like mass spectrometry (MS) based proteomics offer an opportunity for rapid advances. Unfortunately, the enormous amount of MS data generated often remains incompletely analyzed due to a lack of sophisticated bioinformatic tools and field-specific biological expertise for data interpretation. Here we present the initiation of the Archaeal Proteome Project (ArcPP), a community-based effort to comprehensively analyze archaeal proteomes. Starting with the model archaeon Haloferax volcanii, we reanalyze MS datasets from various strains and culture conditions. Optimized peptide spectrum matching, with strict control of false discovery rates, facilitates identifying>72% of the reference proteome, with a median protein sequence coverage of 51%. These analyses, together with expert knowledge in diverse aspects of cell biology, provide meaningful insights into processes such as N-terminal protein maturation, N-glycosylation, and metabolism. Altogether, ArcPP serves as an invaluable blueprint for comprehensive prokaryotic proteomics. While archaeal proteomics advanced rapidly, a comprehensive proteome database for archaea is lacking. Therefore, the authors here launch the Archaeal Proteome Project, a community-effort providing insights into archaeal cell biology via the combined reanalysis of Haloferax volcanii proteomics data.
Item Description:Gesehen am 05.10.2020
Physical Description:Online Resource
ISSN:2041-1723
DOI:10.1038/s41467-020-16784-7

Similar Items