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The nucleoporin MEL-28 promotes RanGTP-dependent γ-tubulin recruitment and microtubule nucleation in mitotic spindle formation

The GTP-bound form of the Ran GTPase (RanGTP), produced around chromosomes, drives nuclear envelope and nuclear pore complex (NPC) re-assembly after mitosis. The nucleoporin MEL-28/ELYS binds chromatin in a RanGTP-regulated manner and acts to seed NPC assembly. Here we show that, upon mitotic NPC di...

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Main Authors: Yokoyama, Hideki (Author) , Koch, Birgit (Author) , Walczak, Rudolf (Author) , Ciray-Duygu, Fulya (Author) , González Sánchez, Juan Carlos (Author) , Devos, Damien (Author) , Mattaj, Iain W. (Author) , Gruss, Oliver (Author)
Format: Article (Journal)
Language:English
Published: 7 Feb 2014
In: Nature Communications
Year: 2014, Volume: 5
ISSN:2041-1723
DOI:10.1038/ncomms4270
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/ncomms4270
Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/ncomms4270
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Author Notes:Hideki Yokoyama, Birgit Koch, Rudolf Walczak, Fulya Ciray-Duygu, Juan Carlos González-Sánchez, Damien P. Devos, Iain W. Mattaj & Oliver J. Gruss
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Summary:The GTP-bound form of the Ran GTPase (RanGTP), produced around chromosomes, drives nuclear envelope and nuclear pore complex (NPC) re-assembly after mitosis. The nucleoporin MEL-28/ELYS binds chromatin in a RanGTP-regulated manner and acts to seed NPC assembly. Here we show that, upon mitotic NPC disassembly, MEL-28 dissociates from chromatin and re-localizes to spindle microtubules and kinetochores. MEL-28 directly binds microtubules in a RanGTP-regulated way via its C-terminal chromatin-binding domain. Using Xenopus egg extracts, we demonstrate that MEL-28 is essential for RanGTP-dependent microtubule nucleation and spindle assembly, independent of its function in NPC assembly. Specifically, MEL-28 interacts with the γ-tubulin ring complex and recruits it to microtubule nucleation sites. Our data identify MEL-28 as a RanGTP target that functions throughout the cell cycle. Its cell cycle-dependent binding to chromatin or microtubules discriminates MEL-28 functions in interphase and mitosis, and ensures that spindle assembly occurs only after NPC breakdown.
Item Description:Gesehen am 09.10.2020
Physical Description:Online Resource
ISSN:2041-1723
DOI:10.1038/ncomms4270

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