A modified dinucleotide for site-specific RNA-labelling by transcription priming and click chemistry
An improved strategy for RNA labelling using an alkyne-carrying dinucleotide is reported. This involves near-quantitative priming by phage RNA-polymerases followed by conjugation of different labels using click chemistry. Moreover, these transcripts bear a ligation compatible 5′-end, and thus throug...
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| Main Authors: | , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
2014
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| In: |
Chemical communications
Year: 2013, Volume: 50, Issue: 11, Pages: 1313-1316 |
| ISSN: | 1364-548X |
| DOI: | 10.1039/C3CC46132G |
| Online Access: | Resolving-System, lizenzpflichtig, Volltext: https://doi.org/10.1039/C3CC46132G Verlag, lizenzpflichtig, Volltext: https://pubs.rsc.org/en/content/articlelanding/2014/cc/c3cc46132g 
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| Author Notes: | Ayan Samanta, André Krause and Andres Jäschke |
| Summary: | An improved strategy for RNA labelling using an alkyne-carrying dinucleotide is reported. This involves near-quantitative priming by phage RNA-polymerases followed by conjugation of different labels using click chemistry. Moreover, these transcripts bear a ligation compatible 5′-end, and thus through ligation the terminal label can be transformed to an internal one. |
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| Item Description: | First published 17 Oct 2013 Gesehen am 09.10.2020 |
| Physical Description: | Online Resource |
| ISSN: | 1364-548X |
| DOI: | 10.1039/C3CC46132G |