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A modified dinucleotide for site-specific RNA-labelling by transcription priming and click chemistry

An improved strategy for RNA labelling using an alkyne-carrying dinucleotide is reported. This involves near-quantitative priming by phage RNA-polymerases followed by conjugation of different labels using click chemistry. Moreover, these transcripts bear a ligation compatible 5′-end, and thus throug...

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Bibliographische Detailangaben
Hauptverfasser: Samanta, Ayan (VerfasserIn) , Krause, André (VerfasserIn) , Jäschke, Andres (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 2014
In: Chemical communications
Year: 2013, Jahrgang: 50, Heft: 11, Pages: 1313-1316
ISSN:1364-548X
DOI:10.1039/C3CC46132G
Online-Zugang:Resolving-System, lizenzpflichtig, Volltext: https://doi.org/10.1039/C3CC46132G
Verlag, lizenzpflichtig, Volltext: https://pubs.rsc.org/en/content/articlelanding/2014/cc/c3cc46132g
Volltext
Verfasserangaben:Ayan Samanta, André Krause and Andres Jäschke
Beschreibung
Zusammenfassung:An improved strategy for RNA labelling using an alkyne-carrying dinucleotide is reported. This involves near-quantitative priming by phage RNA-polymerases followed by conjugation of different labels using click chemistry. Moreover, these transcripts bear a ligation compatible 5′-end, and thus through ligation the terminal label can be transformed to an internal one.
Beschreibung: First published 17 Oct 2013
Gesehen am 09.10.2020
Beschreibung:Online Resource
ISSN:1364-548X
DOI:10.1039/C3CC46132G

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