Proteomic characterization of hippocampus of chronically socially isolated rats treated with fluoxetine: depression-like behaviour and fluoxetine mechanism of action
Due to the severity of depressive symptoms, there remains a necessity in defining the underlying mechanisms of depression and the precise actions of antidepressants in alleviating these symptoms. Proteomics is a powerful and promising tool for discovering novel pathways of cellular responses to dise...
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| Hauptverfasser: | , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
June 2018
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| In: |
Neuropharmacology
Year: 2018, Jahrgang: 135, Pages: 268-283 |
| ISSN: | 1873-7064 |
| DOI: | 10.1016/j.neuropharm.2018.03.034 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.neuropharm.2018.03.034 Verlag, lizenzpflichtig, Volltext: http://www.sciencedirect.com/science/article/pii/S0028390818301461 
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| Verfasserangaben: | Ivana Perić, Victor Costina, Andrijana Stanisavljević, Peter Findeisen, Dragana Filipović |
| Zusammenfassung: | Due to the severity of depressive symptoms, there remains a necessity in defining the underlying mechanisms of depression and the precise actions of antidepressants in alleviating these symptoms. Proteomics is a powerful and promising tool for discovering novel pathways of cellular responses to disease and treatment. As chronic social isolation (CSIS) is a valuable animal model for studying depression, we performed a comparative subproteomic study of rat hippocampus to explore the effect of six weeks of CSIS and the therapeutic effect of chronic fluoxetine (Flx) treatment (last three weeks of CSIS; 15mg/kg/day). Behaviorally, Flx treatment normalized the decreased sucrose preference and increased marble burying results resulting from CSIS, indicative of a FLX-induced attenuation of both anhedonia and anxiety. An analysis of cytosolic and nonsynaptic mitochondrial subproteome patterns revealed that CSIS resulted in down-regulation of proteins involved in mitochondrial transport and energy processes, primarily tricarboxylic acid (TCA) cycle and oxidative phosphorylation. Chronic Flx treatment resulted in an up-regulation of CSIS-altered proteins and additional expression of other transporter and energy-involved proteins. Immunohistochemical analysis revealed hippocampal subregion-specific effects of CSIS and/or Flx treatment on selective protein expressions. |
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| Beschreibung: | Gesehen am 13.10.2020 |
| Beschreibung: | Online Resource |
| ISSN: | 1873-7064 |
| DOI: | 10.1016/j.neuropharm.2018.03.034 |