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An integrated approach for genome annotation of the eukaryotic thermophile Chaetomium thermophilum

The thermophilic fungus Chaetomium thermophilum holds great promise for structural biology. To increase the efficiency of its biochemical and structural characterization and to explore its thermophilic properties beyond those of individual proteins, we obtained transcriptomics and proteomics data, a...

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Bibliographic Details
Main Authors: Bock, Thomas (Author) , Baßler, Jochen (Author) , Koš, Martin (Author) , Hurt, Ed (Author) , Bork, Peer (Author)
Format: Article (Journal)
Language:English
Published: 14 November 2014
In: Nucleic acids research
Year: 2014, Volume: 42, Issue: 22, Pages: 13525-13533
ISSN:1362-4962
DOI:10.1093/nar/gku1147
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1093/nar/gku1147
Verlag, lizenzpflichtig, Volltext: https://academic.oup.com/nar/article/42/22/13525/2411142
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Author Notes:Thomas Bock, Wei-Hua Chen, Alessandro Ori, Nayab Malik, Noella Silva-Martin, Jaime Huerta-Cepas, Sean T. Powell, Panagiotis L. Kastritis, Georgy Smyshlyaev, Ivana Vonkova, Joanna Kirkpatrick, Tobias Doerks, Leo Nesme, Jochen Baßler, Martin Kos, Ed Hurt, Teresa Carlomagno, Anne-Claude Gavin, Orsolya Barabas, Christoph W. Müller, Vera van Noort, Martin Beck and Peer Bork
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Summary:The thermophilic fungus Chaetomium thermophilum holds great promise for structural biology. To increase the efficiency of its biochemical and structural characterization and to explore its thermophilic properties beyond those of individual proteins, we obtained transcriptomics and proteomics data, and integrated them with computational annotation methods and a multitude of biochemical experiments conducted by the structural biology community. We considerably improved the genome annotation of Chaetomium thermophilum and characterized the transcripts and expression of thousands of genes. We furthermore show that the composition and structure of the expressed proteome of Chaetomium thermophilum is similar to its mesophilic relatives. Data were deposited in a publicly available repository and provide a rich source to the structural biology community.
Item Description:Gesehen am 16.10.2020
Physical Description:Online Resource
ISSN:1362-4962
DOI:10.1093/nar/gku1147

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