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An integrated approach for genome annotation of the eukaryotic thermophile Chaetomium thermophilum

The thermophilic fungus Chaetomium thermophilum holds great promise for structural biology. To increase the efficiency of its biochemical and structural characterization and to explore its thermophilic properties beyond those of individual proteins, we obtained transcriptomics and proteomics data, a...

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Bibliographische Detailangaben
Hauptverfasser: Bock, Thomas (VerfasserIn) , Baßler, Jochen (VerfasserIn) , Koš, Martin (VerfasserIn) , Hurt, Ed (VerfasserIn) , Bork, Peer (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 14 November 2014
In: Nucleic acids research
Year: 2014, Jahrgang: 42, Heft: 22, Pages: 13525-13533
ISSN:1362-4962
DOI:10.1093/nar/gku1147
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1093/nar/gku1147
Verlag, lizenzpflichtig, Volltext: https://academic.oup.com/nar/article/42/22/13525/2411142
Volltext
Verfasserangaben:Thomas Bock, Wei-Hua Chen, Alessandro Ori, Nayab Malik, Noella Silva-Martin, Jaime Huerta-Cepas, Sean T. Powell, Panagiotis L. Kastritis, Georgy Smyshlyaev, Ivana Vonkova, Joanna Kirkpatrick, Tobias Doerks, Leo Nesme, Jochen Baßler, Martin Kos, Ed Hurt, Teresa Carlomagno, Anne-Claude Gavin, Orsolya Barabas, Christoph W. Müller, Vera van Noort, Martin Beck and Peer Bork
Beschreibung
Zusammenfassung:The thermophilic fungus Chaetomium thermophilum holds great promise for structural biology. To increase the efficiency of its biochemical and structural characterization and to explore its thermophilic properties beyond those of individual proteins, we obtained transcriptomics and proteomics data, and integrated them with computational annotation methods and a multitude of biochemical experiments conducted by the structural biology community. We considerably improved the genome annotation of Chaetomium thermophilum and characterized the transcripts and expression of thousands of genes. We furthermore show that the composition and structure of the expressed proteome of Chaetomium thermophilum is similar to its mesophilic relatives. Data were deposited in a publicly available repository and provide a rich source to the structural biology community.
Beschreibung:Gesehen am 16.10.2020
Beschreibung:Online Resource
ISSN:1362-4962
DOI:10.1093/nar/gku1147

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